Glucose 6 phosphate (g6p)

Lauric, decanoic and stearic acids, and 5-aminolevulinic acid hydrochloride were purchased from Acros Organics (Fair Lawn, NJ). The 11-hydroxylauric and 12-hydroxylauric-d₂₀ acid standards were purchased from Santa Cruz Biotechnology. The 12-hydroxylauric, palmitic and arachidonic (oil) acids, clotrimazole, imidazole, tert-butyl (tBPH), and cumene hydroperoxides (CuOOH) were obtained from Sigma-Aldrich (St Louis, MO). Myristic acid and econazole were purchased at VWR International, and ketoconazole was from Toronto Research Chemicals. Oleic acid and miconazole were obtained from Thermo Fisher Scientific. Ampicillin, arachidonic acid sodium salt, 18-HETE, 19(S)-HETE, 20-HETE, and 20-HETE-d₆ were all purchased from Cayman Chemical. Isopropyl-β-D-1-thiogalactopyranoside (IPTG), phenylmethanesulfonylfluoride (PMSF), glucose-6-phosphate, and β-nicotinamide adenine dinucleotide phosphate (NADP⁺) were obtained from Alfa Aesar. glucose-6-phosphate dehydrogenase, the spinach Fdx and FdR were purchased from Sigma-Aldrich. All other chemicals and solvents were obtained from standard suppliers and were of reagent or analytical grade.

The DHEA-S standard and ampicillin were purchased from Cayman Chemical. The metabolite standard 16α-hydroxy-DHEA-S was obtained from Steraloids. The IPTG, glucose-6-phosphate, and NADP⁺ were acquired from Alfa Aesar. The glucose-6-phosphate dehydrogenase and the internal standard (IS), estriol 3-sulfate, were purchased from MilliporeSigma. The 1,2-dilauroyl-sn-glycerol-3-phosphocholine, 1,2-dioleoyl-sn-glycero-3-phosphocholine, and 1,2-dilauroyl-sn-glycero-3-phosphoserine were obtained from Avanti Polar Lipids. All other chemicals and solvents used were obtained from standard suppliers and were of reagent or analytical grade.