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Umc 11

Manufactured by Cobioer Biosciences
Sourced in China

The UMC-11 is a compact and versatile laboratory centrifuge designed for routine sample processing. It features a fixed-angle rotor capable of accommodating a variety of tube sizes and volumes. The UMC-11 provides reliable and consistent centrifugation performance to support various laboratory workflows.

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2 protocols using umc 11

1

Cell Line Authentication and Cultivation for Cancer Research

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A549, H1975, HCC827, H2030, and UMC-11 cell lines were obtained from Cobioer Biosciences (Nanjing, China). A549 and H2030 harbor mutations in KRAS; H1975 and HCC827 harbor EGFR mutations. Authentication of A549, HCC827, and H1975 cell lines was performed using short tandem repeat (STR) DNA profiling at Cobioer Biosciences in 2017. Authentication of H2030 and UMC-11 cell lines was performed using STR in 2019. All cell lines were maintained in RPMI-1640 (Hyclone, Omaha, NE, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 100 U/mL penicillin/streptomycin (Hyclone, Logan, UT, USA). All cell lines were passaged for less than 2 months after thawing. For the experiments that required IFN-γ treatment, the cell cultures were supplemented with the indicated amount of IFN-γ (100–2000 IU/mL).
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2

Characterization of Lung Adenocarcinoma Cell Lines

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Human lung adenocarcinoma A549 (ATCC Catalog No. CRL-7909, RRID: CVCL_0023), HCC827 (KCLB Catalog No. 70827, RRID: CVCL_2063), H1975 (ATCC Catalog No. CRL-5908, RRID: CVCL_1511), H2228 (ATCC Catalog No. CRL-5935, RRID: CVCL_1543), H1573 (ATCC Catalog No. CRL-5877, RRID: CVCL_1478), H2444 (ATCC Catalog No. CRL-5945, RRID: CVCL_1552), and UMC-11(ATCC Catalog No. CRL-5975, RRID: CVCL_1784) cell lines were obtained from Cobioer Biosciences. Short tandem repeat (STR) analysis was performed for A549, HCC827, and H1975 cell lines in 2017 and other cell lines in 2019 (Cobioer Biosciences). All of the cell lines were confirmed to be mycoplasma negative (Biothrive Sci. & Tech. Ltd.). Lung adenocarcinoma cells were maintained as a monolayer culture in RPMI1640 (GIBCO) supplemented with 10% FBS (GIBCO) and 1% penicillin/streptomycin (Hyclone).
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