Finger prick blood samples were collected by each participant for the HIV and syphilis rapid testing. The rapid test kits that were used in our study included Colloidal Gold Device Rapid Test for Antibody to Human Immunodeficiency Virus (Beijing Wantai Biological Pharmacy Enterprise Co. Ltd., Beijing, China) and Colloidal Gold Device Rapid Test for Antibody to syphilis (Beijing Wantai Biological Pharmacy Enterprise Co. Ltd., Beijing, China). All participants screening positive for HIV or syphilis were encouraged to undergo confirmation testing at either local CDC or Lingnan Partner Community Support Centre. For those participants confirmed to be HIV positive after confirmation testing, CD4+ T-cell counts were also measured using FACSCalibur flow cytometer (BD-Bioscience, San Jose, CA, USA) based on a single-platform lyse-no-wash procedure using TruCOUNT tubes and TriTEST anti-CD4-FITC-CD8-PE-CD3-PerCP-reagents (BD,USA).
Tritest anti cd4 fitc cd8 pe cd3 percp reagents
Manufactured by BD
Sourced in United States
TriTEST anti-CD4-FITC-CD8-PE-CD3-PerCP-reagents is a set of fluorochrome-conjugated monoclonal antibodies used for the identification and enumeration of T-cell subsets in flow cytometry analysis. The reagents consist of anti-CD4-FITC, anti-CD8-PE, and anti-CD3-PerCP antibodies.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using tritest anti cd4 fitc cd8 pe cd3 percp reagents
1
Rapid HIV and Syphilis Testing Protocol
2
Measuring HIV Viral Load and CD4+ T Cells
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CD4+ T cell counts were measured using a FACSCalibur flow cytometer (BD Bioscience, San Jose, CA, USA) based on a single-platform lyse-no-wash procedure using TruCOUNT tubes and TriTEST anti-CD4-FITC/CD8-PE/CD3-PerCP reagents (BD Bioscience). Viral load (VL) was detected using COBAS AmpliPrep-COBAS AMPLICOR HIV-1 MONITOR Test, version 2.0 (CAP-CA Roche Molecular Systems Inc.) with a detection limit of 20 copies/mL. HIV VL set point was calculated by taking average of all VL values measured during 4–6 months prior to the initiation of HAART. All VL and VL set point values were log10 transformed for data analysis. For HIV-1 subtyping, the pol sequences (entire protease and 256 codons of the RT sequence) were amplified and the edited sequences were aligned against the reference sequences available in the Los Alamos database.
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