Anti caspase 8

Sorafenib was kindly provided by Bayer Pharmaceuticals. YC-1 was obtained from Sigma-Aldrich (Saint Quentin Fallavier, France). Anti-PARP, anti-caspase 8, anti-β-actin, and anti-caspase 9 were obtained from Beyotime (Jiangsu, China). Horseradish peroxidase (HRP)-labeled anti-mouse and anti-rabbit secondary antibodies were from Santa Cruz (Dallas, TX, USA). All other antibodies were purchased from Abcam (Cambridge, TX, USA).

The following primary antibodies were used in the study: rabbit polyclonal anti-LC3B (Cell Signaling Technology, 2775), rabbit polyclonal anti-SQSTM1/p62 (Sigma, SAB2104334), rabbit polyclonal anti-BECN1 (ABclonal, A7353), rabbit polyclonal anti-ATG5 (ABclonal, A0203), mouse monoclonal anti-GAPDH (Beyotime, AG019), rabbit polyclonal anti-caspase-8 (Beyotime, AC056), rabbit polyclonal anti-caspase-9 (Beyotime, AC062), rabbit polyclonal anti-Caspase-3 (ABclonal, A2156), rabbit polyclonal anti-PARP (Beyotime, AP102) and mouse monoclonal anti-CSFV E2 (WH303) (JBT, 9011). Mouse polyclonal anti-CSFV Npro was kindly provided by Dr. Xinglong Yu (Veterinary Department, Hunan Agricultural University, China). The secondary antibodies used for immunoblotting analysis were HRP-conjugated goat anti-mouse IgG (Bioworld, BS12478), HRP-conjugated goat anti-rabbit IgG (Bioworld, BS13278). The secondary antibodies used for immunofluorescence including Dylight 488 goat anti-mouse IgG (EarthOx, E032210), Dylight 488 goat anti-rabbit IgG (EarthOx, E032220), Dylight 549 goat anti-mouse IgG (EarthOx, E032310) and Dylight 549 goat anti-rabbit IgG (EarthOx, E032320).

Supercritical fluid (SCF) extract of Zanthoxylum bungeanum was purchased from Zhengzhou Xuemailong Food Spice Co., Ltd. in Henan Province, China. Both HCT-116 (human colon adenocarcinoma cell line), and HEK293T cells were obtained from the National Collection of Authenticated Cell Cultures in Shanghai, China. McCoy's 5A media, DMEM media, fetal bovine serum (FBS), and 0.25% trypsin solution were obtained from Invitrogen (Carlsbad, CA, USA). The Caspase 8 inhibitor Z-IETD-FMK and P53 inhibitor PFTα hydrobromide were obtained from MedChemExpress (Monmouth Junction, NJ, USA). Primary antibodies (anti-CDK4, anti-cyclin D1, anti-PCNA, anti-BAX, anti-Caspase 3, anti-Cleaved Caspase 3, anti-P53, anti-Bcl-2, anti-Caspase 9, anti-Cleaved Caspase 9, anti-Fas, anti-P21, anti-Caspase 8, anti-Cleaved Caspase 8, and anti-β-actin), anti-mouse, and anti-rabbit secondary were obtained from the Beyotime Institute of Biotechnology (Shanghai, China).