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Rabbit anti n cadherin

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Rabbit anti-N-cadherin is a primary antibody that specifically recognizes the N-cadherin protein. N-cadherin is a transmembrane glycoprotein that mediates calcium-dependent cell-cell adhesion. This antibody can be used in various immunological techniques to detect and analyze the expression of N-cadherin in different cell types and tissues.

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Lab products found in correlation

Rabbit anti vimentin, by Cell Signaling Technology (2 mentions) Oct medium, by Sakura Finetek (2 mentions) Rabbit anti fibronectin, by Merck Group (2 mentions) Fluorescent or bright field imaging microscopy, by Leica (2 mentions) Imagepro plus capture and analysis software, by Media Cybernetics (2 mentions) Apocynin, by Thermo Fisher Scientific (1 mentions) Hrp conjugated goat anti mouse igg, by Bio-Rad (1 mentions) Goat anti rat igg, by Southern Biotech (1 mentions) Mouse anti phospho tyrosine, by Cell Signaling Technology (1 mentions) Goat anti mouse igg1, by Southern Biotech (1 mentions) Hematoxylin, by Vector Laboratories (1 mentions) Hoechst dye, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Mouse anti e cadherin, by BD (1 mentions) Mouse anti ki67, by BD (1 mentions)

Spelling variants of this product

N-cadherin (89 citations) Anti-N-cadherin (26 citations)

4 protocols using rabbit anti n cadherin

1

Immunohistochemical Analysis of Tissue Markers

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Tissues were collected at the designated times and snap-frozen in optimal cutting temperature (OCT) medium (Sakura Finetek, Japan), and 8-μm sections were prepared and stained with the following antibodies; Rabbit anti-fibronectin (1:100) (Millipore, Temecula, CA, USA), rabbit anti-vimentin (1:100) (Cell Signalling Technology, Danvers, MA, USA), rabbit anti-N-cadherin (1:1000) (Thermo Scientific, Rockford, IL, USA), as described previously13 (link),41 (link). Stained sections were analysed using fluorescent or bright-field imaging microscopy (Leica, Germany) and ImagePro Plus Capture and Analysis software (Media Cybernetics, Rockville, MD). Vimentin, Fibronectin and N-Cadherin positive areas were quantified in 15 independent fields/section using Image Pro-Plus software41 (link),44 (link).
Sossey-Alaoui K., Pluskota E., Szpak D., Schiemann W.P, & Plow E.F. (2018). The Kindlin-2 regulation of epithelial-to-mesenchymal transition in breast cancer metastasis is mediated through miR-200b. Scientific Reports, 8, 7360.
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2

Inhibitor and Antibody Validation for Cell Studies

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Inhibitors were as follows: CinnGEL-2ME and Gö-6976 from Biomol, apocynin from Acros Organics. Inhibitors and antibody crosslinking of VCAM-1 did not affect cell viability during the time courses of these studies, consistent with our previous reports [1 (link), 24 (link), 25 (link)]. Antibodies were as follows: Rat anti-mouse VCAM-1 (clone MVCAM.A), mouse anti-human VCAM-1 (clone 51-10C9), rat anti-mouse VE-Cadherin (CD144, cat#550548), rat IgG (isotype antibody, clone R35-95), and FITC-conjugated goat anti-rabbit Ig (cat#554020) [PharMingen]; zenon Alexa Fluor 568-labeled (cat#Z25106, Molecular Probes) rat anti-mouse CD45 (clone I3/2.3), goat anti-mouse IgG1 (cat#1070-01) and goat anti-rat IgG (cat#3050-01) [Southern Biotech]; mouse anti-phosphotyrosine (cat#9411) [Cell Signaling Technology]; Texas Red-conjugated goat anti-rat Ig (cat#R40005) [Caltag]; rabbit anti-human ZO-1 (cat# 41090971), FITC-conjugated rabbit anti-ZO-1 (cat#bs-1329R-FITC)[Bioss], and rabbit anti-phosphoserine (cat#61-8100) [Zymed Lab, Inc]; Alexa 594-conjugated donkey anti rabbit N-Cadherin (cat#150080) [Abcam]; rabbit anti-N-Cadherin (cat#PA5-19486)[Thermo Scientific]; rabbit anti-N-terminal region of angiomotin (cat# ARP34660_P050)[Aviva Systems Biology]; HRP-conjugated donkey anti-rabbit antibody [Amersham Pharmacia]; HRP-conjugated goat anti-mouse IgG [Bio-Rad].
Abdala-Valencia H., Kountz T.S., Marchese M.E, & Cook-Mills J.M. (2018). VCAM-1 induces signals that stimulate ZO-1 serine phosphorylation and reduces ZO-1 localization at lung endothelial cell junctions. Journal of leukocyte biology, 104(1), 215-228.
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3

Immunohistochemical Analysis of Tumor Tissues

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Tumour and lung tissues were harvested and snap-frozen in optimal cutting temperature (OCT) medium (Sakura Finetek, Torrance, CA, USA), and 8-μm sections were prepared. Tumour sections were stained with the following antibodies: rabbit anti-fibronectin (Millipore, Temecula, CA, USA), rabbit anti-vimentin (Cell Signaling Technology, Danvers, MA, USA), rabbit anti-N-cadherin (Thermo Scientific, Rockford, IL, USA), followed by Alexa 488 or Alexa 568-conjugated goat anti-rabbit IgG. Lung sections were stained with hematoxylin (Vector Laboratories, Burlingame, CA, USA) and eosin (Fisher Scientific Co., Kalamazoo, MI, USA) following the manufacturer’s protocol. We used fluorescent or bright-field imaging microscopy (Leica, Wetzlar, Germany) and ImagePro Plus Capture and Analysis software (Media Cybernetics, Rockville, MD, USA) to analyse the stained sections. We used Image Pro-Plus software to quantify stained areas from 10–15 independent fields/sections.
Sossey-Alaoui K., Pluskota E., Szpak D., Schiemann W.P, & Plow E.F. (2018). The Kindlin-2 regulation of epithelial-to-mesenchymal transition in breast cancer metastasis is mediated through miR-200b. Scientific Reports, 8, 7360.
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4

Immunofluorescence Staining of Cell Markers

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Cells were cultured in 8-well chamber glass slides coated with 0.1% gelatin until confluence. They were fixed with 4% paraformaldehyde, rinsed with PBS, and blocked with 3% serum and 1% BSA for immunostaining using following primary antibodies: rabbit anti-ZEB1 (gift from Dr. Douglas Darling, 1:1000)35 (link), mouse anti-Ki67 (BD Pharmingen, Cat# 550609, 1:50), mouse anti-E-cadherin (BD Biosciences, Cat# 610181), rabbit anti-N-cadherin (ThermoFisher, Cat# PA5-19486) together with an anti-rabbit IgG secondary antibody conjugated with Alexa fluor 568 (Invitrogen, Cat# A11011, 1:500), or an anti-mouse IgG second antibody conjugated with Alexa fluor 488 (Invitrogen, Cat# A11001, 1:500). Nuclei were counterstained with Hoechst dye (Invitrogen, Cat# H1399, 1:500), and images were captured by an invert fluorescent microscope.
Chen Y., Lu X., Montoya-Durango D.E., Liu Y.H., Dean K.C., Darling D.S., Kaplan H.J., Dean D.C., Gao L, & Liu Y. (2017). ZEB1 Regulates Multiple Oncogenic Components Involved in Uveal Melanoma Progression. Scientific Reports, 7, 45.
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