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3 protocols using 3 3 diethylthiadicarbocyanine iodide cy5

1

Synthesis of Inorganic Nanoparticles and Dyes

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1-Dodecanthiol (DDT, 98%) was obtained from Acros Organics; Zinc stearate (65%), Zinc oxide (99%) and Indium (III) acetate (In(Ac)3, 98%), Silver (I) nitrate (AgNO3, ≥99.5%), Oleic acid (OA, 90%), 1-Octadecene (ODE, 90%), Oleylamine (OlAm, 70%), 2-Ethylhexanoic acid (99%), Thiourea (99%), Triethyleneglycol dimethylether (98%), L-cysteine hydrochloride monohydrate (98%), Potassium hydroxide (KOH, 90%) were purchased from Sigma Aldrich (Saint Petersburg, Russian Federation). Organic cyanine dyes: 3,3′-diethylthiacarbocyanine iodide (Cy3) (95%) and 3,3′-diethylthiadicarbocyanine iodide (Cy5) (98%) were purchased from Sigma-Aldrich. Solvents: Isopropanol (99.8%), acetone (99.75%), and chloroform (99.9%) were obtained from Vekton (Saint Petersburg, Russian Federation). All chemicals were used without further purification. Ultrapure water (Milli-Q) was used throughout the experiments.
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2

Synthesis of Dye-Functionalized Nanoparticles

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d-penicillamine (99%) and zinc (II) nitrate hexahydrate (99.998%), sodium sulfide nonahydrate (98%) were obtained from Acros Organics (Moscow, Russia); and indium (III) chloride (98%), silver (I) nitrate (≥99.5%), 1-dodecantiol (DDT), 1-octadecene (ODE), oleylamine (OA, 70%), ammonium hydroxide solution (25%), 3-mercaptopropionic acid (MPA, purity > 98%), 2-ethylhexanoic acid (99%), thiourea (99%), triethyleneglycol dimethylether (98%) were purchased from Sigma-Aldrich in Saint Petersburg, Russian Federation. Organic cyanine dyes: 3,3′-diethylthiacarbocyanine iodides (Cy3) and 3,3′-diethylthiadicarbocyanine iodide (Cy5) were purchased from Sigma-Aldrich in Saint Petersburg, Russian Federation. All chemicals were used without further purification. Ultrapure water (Milli-Q) was used throughout the experiments.
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3

Synthesis and Characterization of Luminescent Nanoparticles

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Indium(III) chloride tetrahydrate (InCl3·4H2O), silver nitrate (AgNO3), zinc(II) acetate dihydrate (Zn(CH3COO)2·2H2O), sodium sulfide nonahydrate (Na2S·9H2O), poly(allylamine hydrochloride) (PAH), poly(sodium 4-styrenesulfonate) (PSS), sodium chloride (NaCl), ammonium hydroxide solution (NH4OH), thioglycolic acid (TGA), isopropanol (i-PrOH), 4 nm polystyrene microspheres, 3,3′-diethylthiacarbocyanine iodide (Cy3) and 3,3′-diethylthiadicarbocyanine iodide (Cy5) were purchased from Sigma Aldrich and used without additional purification. All the solutions were prepared using deionized water as a solvent.
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