Cell cycle analyses were performed using a detergent containing hypotonic solution (Krishans reagent) containing 10 µg/ml propidium iodide (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) according to the manufacturers protocol and fluorescence-activated cell sorting (FACS) with a Gallios flow cytometry system (Beckman Coulter, Inc., Brea, CA, USA) assay. Data were analyzed using Kaluza software (version 1.5a; Beckman Coulter, Inc.). Nuclear debris and overlapping nuclei were gated out.
Gallios flow cytometry system
Manufactured by Beckman Coulter
Sourced in United States
The Gallios flow cytometry system is an analytical instrument designed for the identification and quantification of cells, particles, and other biological entities within a sample. It operates by suspending cells or particles in a fluid stream and passing them through a laser beam, which detects the light scattering and fluorescence properties of each individual entity. The system collects and analyzes this data to provide information about the characteristics of the analyzed sample.
Automatically generated - may contain errors
Lab products found in correlation
Kaluza software version 1.5a, by Beckman Coulter (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Fcs express 6, by De Novo Software (1 mentions)
Fxcycle violet stain, by Thermo Fisher Scientific (1 mentions)
Click it plus edu alexa fluor 647 flow cytometry kit, by Thermo Fisher Scientific (1 mentions)
2 protocols using gallios flow cytometry system
1
Cell Cycle Analysis by FACS
2
Cell Cycle and S Phase Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell cycle analysis was performed with FX Cycle violet stain (Invitrogen, Carlsbad, CA, F10347), and S phase analysis was conducted with EdU labeling (Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Kit®, Thermo Fisher Scientific, Waltham, MA, USA). Samples were treated on a Gallios flow cytometry system (Beckman Coulter, Brea, CA, USA)33 . Data were analyzed with FCS Express 6 + software (DeNovo Software®, Glendale, CA, USA).
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