IC50 (50% inhibitory concentration) values for AdSSTR2-yCD-infected MCF-7 cells were determined by using in vitro cytotoxicity assays. AdSSTR2 or AdSSTR2-yCD were administered to cells at 10 or 100 pfu/cell, and the cells were then plated into 96-well plates at a concentration of 5×104 cells per well. After the cells had 1 day to adhere, the media was aspirated and replaced with various concentrations of 5-FC in cell culture media, ranging from 0–1000 µg/mL. After incubating at 37°C for 5 days, the media was aspirated, the wells were washed with PBS, and 100 µL of 2% crystal violet in 70% ethanol were added to each well. The plates were incubated at room temperature for 3h before removing the stain and washing the wells with H2O. Once the wells were dry, the optical density was measured at 540 nm on a Thermo Multiskan MCC Photometer (Fisher Scientific, Houston, TX). Percent survival was determined by dividing the absorbance value of the wells with 5-FC by the absorbance of the wells without 5-FC. The data was entered into GraphPad Prism 4 to generate sigmoidal dose response curves, and IC50 values were calculated from the curves.
Thermo multiskan mcc photometer
Manufactured by Thermo Fisher Scientific
The Thermo Multiskan MCC Photometer is a high-performance microplate reader designed for absorbance measurements. It is capable of performing photometric analyses across a wide range of wavelengths, enabling diverse applications in life science research and clinical diagnostics.
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2 protocols using thermo multiskan mcc photometer
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Cytotoxicity Assay for AdSSTR2-yCD Cells
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Cytotoxicity Assay for AdSSTR2-yCD Cells
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IC50 (50% inhibitory concentration) values for AdSSTR2-yCD-infected MCF-7 cells were determined by using in vitro cytotoxicity assays. AdSSTR2 or AdSSTR2-yCD were administered to cells at 10 or 100 pfu/cell, and the cells were then plated into 96-well plates at a concentration of 5×104 cells per well. After the cells had 1 day to adhere, the media was aspirated and replaced with various concentrations of 5-FC in cell culture media, ranging from 0–1000 µg/mL. After incubating at 37°C for 5 days, the media was aspirated, the wells were washed with PBS, and 100 µL of 2% crystal violet in 70% ethanol were added to each well. The plates were incubated at room temperature for 3h before removing the stain and washing the wells with H2O. Once the wells were dry, the optical density was measured at 540 nm on a Thermo Multiskan MCC Photometer (Fisher Scientific, Houston, TX). Percent survival was determined by dividing the absorbance value of the wells with 5-FC by the absorbance of the wells without 5-FC. The data was entered into GraphPad Prism 4 to generate sigmoidal dose response curves, and IC50 values were calculated from the curves.
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