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Anti pd 1 and anti pd l1

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Anti-PD-1 and anti-PD-L1 are antibodies used in immunoassays. Anti-PD-1 binds to the PD-1 receptor, while anti-PD-L1 binds to the PD-L1 protein. These antibodies can be used to study the interaction between PD-1 and PD-L1, which is involved in the regulation of the immune response.

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Lab products found in correlation

Enhanced chemi luminescence (ecl), by GE Healthcare (2 mentions) Anti β actin, by Merck Group (2 mentions) Bradford protein assay, by Bio-Rad (2 mentions) Anti c myc, by Abcam (2 mentions)

Close spelling variants of this product

Anti-PD-L1 PD-L1 Anti-PD-1

2 protocols using anti pd 1 and anti pd l1

1

Western Blot Analysis of PD-1/PD-L1 Signaling

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Western blotting was performed as described previously [9 (link)]. Briefly, cell lysates were prepared by resuspending cell pellets in 1% NP-40, 10 mM Tris, 140 mM NaCl, 0.1 mM PMSF, 10 mM iodoacetamide, 50 mM NaF, 1 mM EDTA, 0.4 mM sodium orthovanadate, 10 μg/mL leupeptin, 10 μg/mL pepstatin, and 10 μg/mL aprotinin and lysing on ice for 30 min. Cell lysates were centrifuged at 12,000 × g for 15 min to remove nuclei and cell debris. Protein concentration of the soluble extracts was determined by using the Bradford protein assay (Bio-Rad). Fifty micrograms of protein (per lane) was separated by 10% SDS-PAGE and transferred to PVDF membranes, which were probed for indicated antibody: anti-PD-1 and anti-PD-L1 (Cell Signaling Technology); anti-c-Myc (Abcam); and anti-beta-actin (Sigma-Aldrich). Proteins were detected with ECL (GE Healthcare Amersham).
Cheng P., Eksioglu E.A., Chen X., Kandell W., Le Trinh T., Cen L., Qi J., Sallman D.A., Zhang Y., Tu N., Adams W.A., Zhang C., Liu J., Cleveland J.L., List A.F, & Wei S. (2019). S100A9-induced overexpression of PD-1/PD-L1 contributes to ineffective hematopoiesis in myelodysplastic syndromes. Leukemia, 33(8), 2034-2046.
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2

Western blotting analysis of PD-1, PD-L1, and c-Myc

Check if the same lab product or an alternative is used in the 5 most similar protocols
Western blotting was performed as described previously.9 (link) Briefly, cell lysates were prepared by resuspending cell pellets in 1% NP-40, 10 mM Tris, 140 mM NaCl, 0.1 mM PMSF, 10 mM iodoacetamide, 50 mM NaF, 1 mM EDTA, 0.4 mM sodium orthovanadate, 10 μg/mL leupeptin, 10 μg/mL pepstatin, and 10 μg/mL aprotinin and lysing on ice for 30 min. Cell lysates were centrifuged at 12,000 × g for 15 min to remove nuclei and cell debris. Protein concentration of the soluble extracts was determined by using the Bradford protein assay (Bio-Rad). Fifty micrograms of protein (per lane) were separated by 10% SDS-PAGE and transferred to PVDF membranes, which were probed for indicated antibody: anti-PD-1 and anti-PD-L1 (Cell Signaling Technology); anti-c-Myc (Abcam); and anti-beta-actin (Sigma-Aldrich). Proteins were detected with ECL (GE Healthcare Amersham).
Cheng P., Eksioglu E.A., Chen X., Kandell W., Le Trinh T., Cen L., Qi J., Sallman D.A., Zhang Y., Tu N., Adams W.A., Zhang C., Liu J., Cleveland J.L., List A.F, & Wei S. (2019). S100A9-induced overexpression of PD-1/PD-L1 contributes to ineffective hematopoiesis in myelodysplastic syndromes. Leukemia, 33(8), 2034-2046.
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