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Mouse glucagon elisa

Manufactured by Crystal Chem
Sourced in United States

The Mouse Glucagon ELISA is a quantitative sandwich enzyme-linked immunosorbent assay designed for the measurement of mouse glucagon levels in biological samples such as plasma, serum, and cell culture supernatants. It utilizes a pair of highly specific antibodies to capture and detect the target analyte.

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2 protocols using mouse glucagon elisa

1

Fasting Hormone Levels and GLP-1 Antagonism

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Plasma hormones were measured following a 6-hour fast between 7 am and 1 pm, using Ultra-Sensitive Mouse Insulin ELISA (Crystal Chem, 90080), Mouse Glucagon ELISA (Crystal Chem, 81518), and Total GLP-1 (ver. 2) (Mesoscale, K150JVC-1).
Glp1 receptor antagonism: Exendin 9-39 amide (Sigma Aldrich, E7269) was injected s.c. daily during pregnancy at a concentration of 25 nmol/kg in 0.9% NaCl, normalized to 10 mL/kg.
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2

Mouse Plasma Insulin and Glucagon ELISA

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Insulin and glucagon concentrations were measured in mouse plasma using a Mouse Ultrasensitive Insulin ELISA (ALPCO, Salem, NH, USA) and a Mouse Glucagon ELISA (Crystal Chem, Zaandam, Netherlands) according to the manufacturer’s instructions.
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