Monoclonal antibodies specific for beclin 1 and p62

Manufactured by Merck Group

Sourced in United States

Monoclonal antibodies specific for Beclin-1 and p62 are laboratory reagents designed for use in research applications. These antibodies are used to detect and quantify the expression of the Beclin-1 and p62 proteins, which are involved in cellular processes such as autophagy. The core function of these monoclonal antibodies is to provide a tool for researchers to study the roles of Beclin-1 and p62 in various biological systems.

Automatically generated - may contain errors

Lab products found in correlation

Complete protease inhibitor cocktail, by Roche (3 mentions) Anti atg12, by Santa Cruz Biotechnology (3 mentions) Anti bcl 2, by Santa Cruz Biotechnology (3 mentions) Anti map lc3, by Santa Cruz Biotechnology (3 mentions) Anti bax, by Santa Cruz Biotechnology (3 mentions) Anti active caspase 3, by Cell Signaling Technology (2 mentions) Anti sirt1, by Cell Signaling Technology (2 mentions) Anti parkin, by Cell Signaling Technology (2 mentions) Immun star hrp substrate kit, by Bio-Rad (2 mentions) Anti nlrp3 antibody, by Adipogen (1 mentions) Anti catalase, by Santa Cruz Biotechnology (1 mentions) Anti mnsod, by Santa Cruz Biotechnology (1 mentions) Ppi3k and pi3k, by Cell Signaling Technology (1 mentions) P mtor and mtor, by Cell Signaling Technology (1 mentions) Immun star horseradish peroxidase hrp substrate kit, by Bio-Rad (1 mentions)

3 protocols using monoclonal antibodies specific for beclin 1 and p62

Antibody Selection for Molecular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal antibodies specific for Beclin-1 and p62 were purchased from Sigma-Aldrich (Saint Louis, USA). Anti-GAPDH monoclonal antibody was acquired from Calbiochem-Merck Chemicals Ltd. (Nottingham, UK). Similarly, anti-NLRP3 antibody was purchased from Adipogen (San Diego, USA); while anti-active caspase-3, anti-SIRT-1 and anti-Parkin were obtained from Cell Signalling Technology (Beverly, MA, USA). Finally, anti-IL-1β (p17), anti-OGG-1, anti-Bcl-2, anti-Bax, anti-MnSOD, anti-catalase, anti-ATG12 and anti-MAP-LC3 antibodies from (Santa Cruz Biotechnology). A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim (Indianapolis, IN). The Immun Star HRP substrate kit was obtained from Bio-Rad Laboratories Inc. (Hercules, CA).

Pavillard L.E., Cañadas-Lozano D., Alcocer-Gómez E., Marín-Aguilar F., Pereira S., Robertson A.A., Muntané J., Ryffel B., Cooper M.A., Quiles J.L., Bullón P., Ruiz-Cabello J, & Cordero M.D. (2017). NLRP3-inflammasome inhibition prevents high fat and high sugar diets-induced heart damage through autophagy induction. Oncotarget, 8(59), 99740-99756.

+ Open protocol

+ Expand

Antibody Detection for Autophagy Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal antibodies specific for Beclin‐1 and p62 were purchased from Sigma‐Aldrich. The anti‐GAPDH monoclonal antibody was acquired from Calbiochem‐Merck Chemicals Ltd. Similarly, anti‐active caspase‐3, anti‐SIRT‐1, p‐PI3K and PI3K, p‐mTOR and mTOR, and anti‐Parkin were obtained from Cell Signaling Technology. Finally, anti‐Bcl‐2, anti‐Bax, anti‐ATG12, and anti‐MAP‐LC3 antibodies were obtained from Santa Cruz Biotechnology. A cocktail of protease inhibitors (Complete™ Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim. The Immun Star HRP substrate kit was obtained from Bio‐Rad Laboratories Inc.

Marín‐Aguilar F., Lechuga‐Vieco A.V., Alcocer‐Gómez E., Castejón‐Vega B., Lucas J., Garrido C., Peralta‐Garcia A., Pérez‐Pulido A.J., Varela‐López A., Quiles J.L., Ryffel B., Flores I., Bullón P., Ruiz‐Cabello J, & Cordero M.D. (2019). NLRP3 inflammasome suppression improves longevity and prevents cardiac aging in male mice. Aging Cell, 19(1), e13050.

+ Open protocol

+ Expand

Monoclonal Antibodies in Beclin 1 and p62 Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monoclonal antibodies specific for Beclin 1 and p62 were purchased from Sigma-Aldrich (Saint Louis, USA). The anti–glyceraldehyde-3-phosphate dehydrogenase monoclonal antibody was acquired from Calbiochem-Merck Chemicals Ltd. (Nottingham, UK). Last, anti–Bcl-2, anti-Bax, anti-ATG12, and anti–MAP-LC3 antibodies were obtained from Santa Cruz Biotechnology. Anti-AMH antibody was obtained from Abbexa (Cambridge, UK). NLRP3 inhibitors MCC950 and 16673-34-0 were obtained from Sigma-Aldrich (Saint Louis, USA) and R&D Systems (Minneapolis, USA), respectively. A cocktail of protease inhibitors (cOmplete Protease Inhibitor Cocktail) was purchased from Boehringer Mannheim (Indianapolis, IN). The Immun-Star horseradish peroxidase (HRP) substrate kit was obtained from Bio-Rad Laboratories Inc. (Hercules, CA).

Navarro-Pando J.M., Alcocer-Gómez E., Castejón-Vega B., Navarro-Villarán E., Condés-Hervás M., Mundi-Roldan M., Muntané J., Pérez-Pulido A.J., Bullon P., Wang C., Hoffman H.M., Sanz A., Mbalaviele G., Ryffel B, & Cordero M.D. (2021). Inhibition of the NLRP3 inflammasome prevents ovarian aging. Science Advances, 7(1), eabc7409.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!