Before treatment, HepG2 and HLE cells were cultured in serum-free medium for 12 h to arrest the cell cycle, then the serum-free supernatant was replaced by fresh medium containing 10% FCS. The cells were transferred into 12-well plates, treated with NaHS and rapamycin for 24 h, then stained with propidium iodide (PI) at a final concentration of 50 mg/l. DNA content was analyzed with a FACScan-420 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA). The distribution of cells in different cell cycle stages was determined according to the DNA content.
Facscan 420 flow cytometer
Manufactured by BD
Sourced in United States
The BD FACScan-420 is a flow cytometer designed for cell analysis. It uses laser excitation and sensitive detectors to measure the physical and fluorescent characteristics of particles or cells in a fluid stream. The FACScan-420 provides accurate data on parameters such as cell size, granularity, and multiple fluorescent markers.
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Lab products found in correlation
2 protocols using facscan 420 flow cytometer
1
Cell Cycle Arrest and Analysis
2
Flow Cytometric Analysis of Apoptosis
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Cell apoptosis was measured by FCM analysis using Annexin V-FITC/PI Apoptosis Detection Kit (40302ES20, Yeasen, Shanghai, China). Briefly, cells were collected and incubated with 5 µL Annexin V-FITC and 10 µL PI for 15 min at room temperature. Adjusted the volume to 500 µL with binding buffer. The apoptosis of HCC cells was analyzed with a FACScan-420 flow cytometer (BD Biosciences, Franklin Lakes, NJ, USA).
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