Genomic dna ffpe one step kit for diatech magcore hf16plus extractor

DNA samples were extracted from tumor-enriched areas using either the Genomic DNA FFPE One-Step Kit for Diatech MagCore^® HF16Plus extractor (RBC Bioscience, New Taipei City, Taiwan) or the QIAamp DNA FFPE Tissue kit (Qiagen, Valencia, CA, USA) and/or the DNeasy Blood & Tissue Kit for DNA from fresh tissue (FT) or primary melanoma cell lines, according to the manufacturer’s instructions.

All diagnoses were performed by two expert dermatopathologists (FC and MG), and subsequently reviewed for tumor content, neoplastic cell morphology (epithelioid vs spindled) and immunohistochemistry evaluation (LM and FG).
The tumor area was identified, and marked by the pathologists, on hematoxylin and eosin stained slides. Marked tumor tissue was manually dissected from two to six slides (8-micron thick) of FFPE tissue for each case.
Genomic DNA was extracted from the sections using the QIAmp DNA FFPE tissue kit (Qiagen, Valencia, CA, USA) or the Genomic DNA FFPE One-Step Kit for Diatech MagCore® HF16Plus extractor (RBC Bioscience, New Taipei City, Taiwan), in both cases according to the manufacturer's instructions. Quality and concentration of the DNA samples were examined by SPECTROstar Nano (BMG Labtech, Offenburg, Germany).