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Fitc conjugated igg2a

Manufactured by BD

FITC-conjugated IgG2a is a laboratory reagent consisting of fluorescein isothiocyanate (FITC) conjugated to an immunoglobulin G2a (IgG2a) antibody. It can be used for various immunological and cell biology applications that require fluorescent labeling.

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3 protocols using fitc conjugated igg2a

1

Long-term Immunophenotyping of hBM-MSCs

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hBM-MSC immunophenotypes were evaluated during long-term culture25 (link). Harvested cells were labeled using mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD105, CD90, CD73, CD34, CD45, CD11b and CD79a, and fluorescein isothiocyanate (FITC)-conjugated HLA-DR (BD Biosciences, San Jose, CA, USA). As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension containing 1 × 106 cells was incubated with monoclonal antibodies for 15 min at room temperature in the dark and fixed with BD Cytofix (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuest Proversion 6.0 software (BD Biosciences).
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2

Immunophenotyping of Stem Cells

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Immunophnotypes were evaluated using mouse anti-human monoclonal antibodies: fluorescein isothiocyanate (FITC)-conjugated HLA-DR (BD Biosciences, San Jose, CA) and Phycoerythin (PE)-conjugated CD73, CD90, CD105, CD11b, CD34, CD45 and CD79a. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension containing 1 × 106 cells was incubated with monoclonal antibodies for 15 minutes at room temperature in the dark and fixed with BD CytofixTM (BD Biosciences). The analysis was performed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuestTM Proversion 6.0 software (BD Biosciences).
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3

Immunophenotyping of Mesenchymal Stem Cells

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Cells harvested from 3rd passage (P3) were used for immunophenotyping analysis. Mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD73, CD90, CD105, CD45, CD11b, CD34, CD79a, and FITC-conjugated HLA-DR (BD Biosciences, San Jose, CA) were used. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension, containing 1 × 106 cells, were incubated with monoclonal antibodies at room temperature in the dark and fixed with BD CytofixTM (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuestTM Proversion 6.0 software (BD Biosciences).
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