Fitc conjugated igg2a

hBM-MSC immunophenotypes were evaluated during long-term culture^{25 (link)}. Harvested cells were labeled using mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD105, CD90, CD73, CD34, CD45, CD11b and CD79a, and fluorescein isothiocyanate (FITC)-conjugated HLA-DR (BD Biosciences, San Jose, CA, USA). As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension containing 1 × 10⁶ cells was incubated with monoclonal antibodies for 15 min at room temperature in the dark and fixed with BD Cytofix (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuest Proversion 6.0 software (BD Biosciences).

Immunophnotypes were evaluated using mouse anti-human monoclonal antibodies: fluorescein isothiocyanate (FITC)-conjugated HLA-DR (BD Biosciences, San Jose, CA) and Phycoerythin (PE)-conjugated CD73, CD90, CD105, CD11b, CD34, CD45 and CD79a. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension containing 1 × 10⁶ cells was incubated with monoclonal antibodies for 15 minutes at room temperature in the dark and fixed with BD Cytofix^TM (BD Biosciences). The analysis was performed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuest^TM Proversion 6.0 software (BD Biosciences).

Cells harvested from 3rd passage (P3) were used for immunophenotyping analysis. Mouse anti-human monoclonal antibodies: phycoerythrin (PE)-conjugated CD73, CD90, CD105, CD45, CD11b, CD34, CD79a, and FITC-conjugated HLA-DR (BD Biosciences, San Jose, CA) were used. As a control, isotype PE-conjugated IgG1 and FITC-conjugated IgG2a (BD Biosciences) were used. The cell suspension, containing 1 × 10⁶ cells, were incubated with monoclonal antibodies at room temperature in the dark and fixed with BD Cytofix^TM (BD Biosciences). The samples were analyzed on FACSCalibur cytometer (BD Biosciences) and the resulting data were processed using CellQuest^TM Proversion 6.0 software (BD Biosciences).