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Taqman r fast universal pcr master mix for taqman assays

Manufactured by Thermo Fisher Scientific
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TaqMan(R) Fast Universal PCR Master Mix is a ready-to-use solution for real-time PCR assays using TaqMan technology. It contains all the necessary components, including DNA polymerase, nucleotides, and buffer, to perform fast and efficient real-time PCR reactions.

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Lab products found in correlation

Trizol, by Thermo Fisher Scientific (2 mentions) Stepone real time pcr system, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

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2 protocols using taqman r fast universal pcr master mix for taqman assays

1

Quantitative RT-PCR Analysis of Pancreatic Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from cultured human islets and INS-1E cells using TRIzol (Invitrogen), and RT-PCR performed as described previously 67 . For analysis, we used the Applied Biosystems StepOne Real-Time PCR system (Applied Biosystems, CA, USA) with TaqMan(R) Fast Universal PCR Master Mix for TaqMan assays (Applied Biosystems). TaqMan(R) Gene Expression Assays were used for pdx1 (Hs00426216_m1), SLC2A2 (Hs01096905_m1), GCK (Hs01564555_m1), insulin (Hs02741908_m1), PPIA (Hs99999904_m1), BCL2L11 (Hs01083836_m1) and tubulin (Hs00362387_m1) for human and PDX1 (Rn00755591_m1), SLC2A2 (Rn00563565_m1), GCK (Rn00688285_m1), INS1 (Rn02121433_g1), INS2 (Rn01774648_g1), PPIA (Rn00690933_m1) and tuba1a (Rn01532518_g1) for rat.
Ardestani A., Paroni F., Azizi Z., Kaur S., Khobragade V., Yuan T., Frogne T., Tao W., Oberholzer J., Pattou F., Conte J.K, & Maedler K. (2014). MST1 is a novel regulator of apoptosis in pancreatic beta-cells. Nature medicine, 20(4), 385-397.
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2

Quantitative RT-PCR Analysis of Pancreatic Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was isolated from cultured human islets and INS-1E cells using TRIzol (Invitrogen), and RT-PCR performed as described previously 67 . For analysis, we used the Applied Biosystems StepOne Real-Time PCR system (Applied Biosystems, CA, USA) with TaqMan(R) Fast Universal PCR Master Mix for TaqMan assays (Applied Biosystems). TaqMan(R) Gene Expression Assays were used for pdx1 (Hs00426216_m1), SLC2A2 (Hs01096905_m1), GCK (Hs01564555_m1), insulin (Hs02741908_m1), PPIA (Hs99999904_m1), BCL2L11 (Hs01083836_m1) and tubulin (Hs00362387_m1) for human and PDX1 (Rn00755591_m1), SLC2A2 (Rn00563565_m1), GCK (Rn00688285_m1), INS1 (Rn02121433_g1), INS2 (Rn01774648_g1), PPIA (Rn00690933_m1) and tuba1a (Rn01532518_g1) for rat.
Ardestani A., Paroni F., Azizi Z., Kaur S., Khobragade V., Yuan T., Frogne T., Tao W., Oberholzer J., Pattou F., Conte J.K, & Maedler K. (2014). MST1 is a novel regulator of apoptosis in pancreatic beta-cells. Nature medicine, 20(4), 385-397.
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