Legendplex mouse cytokine release syndrome panel 13 plex multi analyte flow assay kit

Manufactured by BioLegend

The LEGENDplex Mouse Cytokine Release Syndrome Panel (13-plex) Multi-Analyte Flow Assay Kit is a bead-based immunoassay designed to measure the concentration of 13 mouse cytokines and chemokines in a single sample. The kit utilizes fluorescent-coded beads and a flow cytometer to quantify the target analytes simultaneously.

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Lab products found in correlation

Legendplex quognit software, by BioLegend (2 mentions) Microvettecb 300 lh, by Sarstedt (2 mentions)

Spelling variants of this product

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2 protocols using legendplex mouse cytokine release syndrome panel 13 plex multi analyte flow assay kit

Cytokine Release Syndrome Panel Quantification

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Plasma was acquired from blood collected by cardiac puncture into heparin-coated capillary tubes (Microvette CB 300 Lh, Sarstedt). Ascites fluid was collected from mice, centrifuged at 2,000 rpm for 15 minutes, and supernatant was frozen at −80°C until assay. Ascites supernatant and plasma samples were diluted 1:2 in assay buffer and assayed according to the manufacturer's protocol for the LEGENDplex Mouse Cytokine Release Syndrome Panel (13-plex) Multi-Analyte Flow Assay Kit (BioLegend). Samples were acquired in duplicate the same day of staining, on a BD LSR Fortessa flow cytometer and analyzed using LEGENDplex Quognit software (BioLegend).

Rodriguez G.M., Galpin K.J., Cook D.P., Yakubovich E., Maranda V., Macdonald E.A., Wilson-Sanchez J., Thomas A.L., Burdette J.E, & Vanderhyden B.C. (2022). The Tumor Immune Profile of Murine Ovarian Cancer Models: An Essential Tool for Ovarian Cancer Immunotherapy Research. Cancer Research Communications, 2(6), 417-433.

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Multiplex Cytokine Analysis in Ascites and Plasma

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Plasma was acquired from blood collected by cardiac puncture into heparin coated capillary tubes (Microvette CB 300 Lh, Sarstedt). Ascites fluid was collected from mice, centrifuged at 2000 rpm for 15 minutes, and supernatant was frozen at −80°C until assay. Ascites supernatant and plasma samples were diluted 1:2 in assay buffer and assayed according to the manufacturer’s protocol for the LEGENDplex^™ Mouse Cytokine Release Syndrome Panel (13-plex) Multi-Analyte Flow Assay Kit (BioLegend). Samples were acquired in duplicate the same day of staining, on a BD LSR Fortessa^™ flow cytometer and analyzed using LEGENDplex Quognit software (Biolegend).

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