Cell lysates were subjected to Western blot analysis using specific antibodies to CD63 (Santa Cruz Biotechnology, Dallas, Tx, USA), TSG101 (Santa Cruz Biotechnology), tenascin (TNC) (Sigma), fibrinogen-β (FGB) (Santa Cruz Biotechnology), phospho-NF-κB p65 (Ser536) (Cell Signaling Technology, CST, Denvers, MA, USA), and NF-κB p65 (CST). The blot was reprobed with actin-horseradish peroxidase (HRP) antibody (Santa Cruz Biotechnology) to compare protein load in each lane. Densitometry analysis was done using ImageJ software (National Institutes of Health, Bethesda, MD).
Fibrinogen β fgb
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Fibrinogen-β (FGB) is a protein that is a component of the fibrinogen complex, which is a key factor in the blood clotting process. FGB plays a crucial role in the formation of fibrin, the insoluble protein that forms the structural matrix of blood clots. This protein is commonly used in research settings to study the mechanisms of blood coagulation and thrombosis.
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Lab products found in correlation
2 protocols using fibrinogen β fgb
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Western Blot Analysis of Extracellular Vesicles
2
Extracellular Vesicle Protein Analysis
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The copyright holder for this preprint this version posted February 9, 2021. ; https://doi.org/10.1101/2021.02.08.430369 doi: bioRxiv preprint Cells lysates were subjected to Western blot analysis using specific antibodies to CD63 (Santa Cruz Biotechnology), TSG101 (Santa Cruz Biotechnology), tenascin (TNC) (Sigma), fibrinogenβ (FGB) (Santa Cruz Biotechnology), phospho-NF-kB p65 (Ser536) (Cell Signaling Technology, CST), NF-kB p65 (CST). The blot was reprobed with actin-HRP antibody (Santa Cruz Biotechnology) to compare protein load in each lane. Densitometry analysis was done using Image J software.
The copyright holder for this preprint this version posted February 9, 2021. ; https://doi.org/10.1101/2021.02.08.430369 doi: bioRxiv preprint Cells lysates were subjected to Western blot analysis using specific antibodies to CD63 (Santa Cruz Biotechnology), TSG101 (Santa Cruz Biotechnology), tenascin (TNC) (Sigma), fibrinogenβ (FGB) (Santa Cruz Biotechnology), phospho-NF-kB p65 (Ser536) (Cell Signaling Technology, CST), NF-kB p65 (CST). The blot was reprobed with actin-HRP antibody (Santa Cruz Biotechnology) to compare protein load in each lane. Densitometry analysis was done using Image J software.
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