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Kac1591

Manufactured by Thermo Fisher Scientific
Sourced in United States

The KAC1591 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor that can accommodate various sample types and volumes. The centrifuge provides consistent speed and temperature control to ensure reliable and reproducible results.

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2 protocols using kac1591

1

Multiplex Cytokine Profiling by ELISA

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The corresponding ELISA kits were used to detect serum inflammatory cytokines including TNF-α (Invitrogen, KAC1751, USA, sensitivity of 3 pg/mL, range of 15-1500 pg/mL), IL-1β (Invitrogen, BMS224HS, sensitivity of 0.05 pg/mL, range of 0.16-10.0 pg/mL), IL-6 (Invitrogen, EH2IL6, sensitivity <1 pg/mL, range of 10.24-400 pg/mL), IL-8 (Invitrogen, KAC1301, sensitivity of 0.7 pg/mL, range of 7-750 pg/mL), IL-10 (Invitrogen, KHC0101, sensitivity <1 pg/mL, range of 7.8-500 pg/mL), and IL-17A (Invitrogen, KAC1591, sensitivity of 2 pg/mL, range of 15-1000 pg/mL). The procedures of ELISA assay were in accordance with the guideline from the manufacturer.
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2

Cytokine Profiling in Cell-Free Sputum Supernatants

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In cell-free sputum supernatants, the following cytokines were measured with ELISA kits according to the manufacturer's instructions: IL-1β (ab46052; Abcam, Cambridge, UK), IL-5 (ab46026; Abcam), IL-6 (KHC0061; Invitrogen, Camarillo, CA, USA), IL-8 (ab46032; Abcam), IL-13 (ab46038; Abcam), IL-17 (KAC1591; Invitrogen), IL-25 (EK0793; Boster Biotech; Pleasanton, CA, USA), IL-33 (SEB980Hu; Cloud-Clone Corp., Katy, TX, USA), TNF-α (ab46087; Abcam), TSLP (DTSLP0; R&D Systems, Minneapolis, MN, USA), GM-CSF (HCYTOMAG-60K; EMD Millipore, Darmstadt, Germany), and IFN-γ (HCYTOMAG-60K; EMD Millipore). Cytokine levels were normalized to protein concentrations in the sputum.
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