Elisa kits for tnf α

Tuna was purchased from Zhejiang Rongchuang Food Co. LTD (Zhoushan, China). DSS (MW:36000) from MP Biomedicals (San Mateo, CA, USA). ELISA kits for TNF-α, IL-6 and LPS were purchased from Nanjing Jiancheng Institute of Biological Engineering (Nanjing, China) and Wuhan Boster Company (Wuhan, China), respectively. DAO, GSH-PX, and SOD detection kits were purchased from Wuhan Huamei Biology Co., LTD (Wuhan, China). Trizol Reagent, the cDNA Reverse transcription Kit, and the TB Green^TM Premix Ex TapTM Fluorescent Quantitative Kit were purchased from TaKaRa (Dalian, China). Fecal genomic DNA extraction kits were obtained from TianGen Biotechnology (Beijing, China). All other chemical reagents are commercially available analytical grade.

AL-1 was previously synthesized and purified in our laboratory. Trinitrobenzenesulfonic acid (TNBS) was purchased from Sigma-Aldrich (St. Louis, MO). Kits for determining myeloperoxidase (MPO) and ELISA kits for TNF-α, IL-1β and IL-6 were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Antibodies against p65, p-p65, IκBα, p-IκBα, COX-2 and PPAR-γ were purchased from Cell Signaling Technology Inc (Boston, MA). Antibodies against p50 and β-actin were purchased from Abcam (Cambridge, MA, USA). All other reagents were obtained from Sigma Chemical Co. (St. Louis, MO).