Human 17 plex panel

Participants were enrolled between August 2013 and July 2015. They completed a sociodemographic survey, including self-reported substance use, and behavioral interview, mental health and neurological screeners (e.g., International HIV Dementia Scale-IHDS (Sacktor et al, 2005 (link))), neuropsychological test battery (Rubin et al, 2019 (link)), functional status assessments (e.g., Patient Assessment of Own Functioning Inventory-PAOFI (Richardson-Vejlgaard et al, 2009 (link)), a neuromedical evaluation by a Ugandan medical officer, and peripheral blood draw to assess HIV status (CD4 cell count, HIV RNA). A subset of participants (n=61) consented to receive an optional lumbar puncture at enrollment, and CSF levels of 17 cytokines/chemokines (Human 17-Plex Panel, Bio-Rad, Hercules, CA) and 20 biomarkers of neurodegeneration (Milliplex Catalog: HMMP1-55K-03; HMMP2-55K-05; HNDG4MAG-36K-05; HND1MAG-39K-07; HNDG3MAG-36K-10) were measured by multiplex assays using with the Luminex-200® system in combination with Luminex manager software (Bioplex manager 5·0, Bio-Rad, Hercules, CA) (Abassi et al, 2017 (link)).

Participants were enrolled between August 2013 and July 2015 and completed a sociodemographic and behavioral interview, mental health screen (Center for Epidemiologic Studies Depression Scale [CES-D]) (Radloff 1977 ), functional assessments (Patient Assessment of Own Functioning Inventory [PAOFI] (Richardson-Vejlgaard et al. 2009 (link)), Instrumental Activities of Daily living [IADL] (Fieo et al. 2010 (link)), Karnofsky functional status score (Karnofsky et al. 1948 ), Bolton Functional assessment (Bolton et al. 2004 (link))), and a dementia screener (International HIV Dementia Scale [IHDS] (Sacktor et al. 2005 (link))). Participants underwent a neuromedical evaluation and peripheral blood draw to confirm HIV status, CD4 cell count, HIV RNA, fasting glucose and lipids, and a metabolic panel. A subset of participants consented to receive an optional lumbar puncture at enrollment and CSF was used to measure 17 cytokines/chemokines via multiplex profiling with the Luminex Platform (Human 17-Plex Panel, Bio-Rad, Hercules, CA) and 20 biomarkers of neurodegenerative breakdown products (Milliplex Catalog: HMMP1–55 K-03; HMMP2–55 K-05; HNDG4MAG-36 K-05; HND1MAG-39 K-07; HNDG3MAG-36 K-10) (Abassi et al. 2017 ).