Log In Sign up
The largest database of trusted experimental protocols

Multitox glo

Manufactured by Promega
Visit Supplier

MultiTox Glo is a multiplex assay system that allows for the simultaneous measurement of cell viability and cytotoxicity in the same cell population. The core function of this product is to provide a quantitative assessment of cell health status.

Automatically generated - may contain errors

Lab products found in correlation

Celltiter glo reagent, by Agilent Technologies (2 mentions) Synergy 4, by Agilent Technologies (2 mentions) Celltiter glo, by Promega (2 mentions)

Close spelling variants of this product

MultiTox-Glo Multiplex Cytotoxicity Assay (6 citations) MultiTox-Glo Multiplex CytotoxicityKit (4 citations) MultiTox-Glo Multiplex Cytotoxicity Assay Kit (3 citations)

2 protocols using multitox glo

1

Evaluating Cell Viability Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Viability was evaluated using a commercially available chemiluminescent reagents (CellTiter-Glo, Promega Corp. G7572; MultiTox Glo, Promega Corp. G9270) in 96-well plate format according to the manufacturers protocol. Cells were exposed to either vehicle, V-9302, CB-839, V-9302 + chloroquine (CLQ), or CB-839 + CLQ and incubated for a period of 48 h. Subsequently, CellTiter-Glo reagent was added and the plates were read using a plate reader (BioTek Synergy 4) with standard settings. Each set of conditions was replicated at least three times, technically and biologically. Error is reported as standard deviation (SD). The sulforhodamine B assay was run analogously to the above assays as previously described19 (link).
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
+ Open protocol
+ Expand
2

Evaluating Cell Viability Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
Viability was evaluated using a commercially available chemiluminescent reagents (CellTiter-Glo, Promega Corp. G7572; MultiTox Glo, Promega Corp. G9270) in 96-well plate format according to the manufacturers protocol. Cells were exposed to either vehicle, V-9302, CB-839, V-9302 + chloroquine (CLQ), or CB-839 + CLQ and incubated for a period of 48 h. Subsequently, CellTiter-Glo reagent was added and the plates were read using a plate reader (BioTek Synergy 4) with standard settings. Each set of conditions was replicated at least three times, technically and biologically. Error is reported as standard deviation (SD). The sulforhodamine B assay was run analogously to the above assays as previously described19 (link).
Schulte M.L., Fu A., Zhao P., Li J., Geng L., Smith S.T., Kondo J., Coffey R.J., Johnson M.O., Rathmell J.C., Sharick J.T., Skala M.C., Smith J.A., Berlin J., Washington M.K., Nickels M.L, & Manning H.C. (2018). Pharmacological Blockade of ASCT2-dependent Glutamine Transport Leads To Anti-tumor Efficacy in Preclinical Models. Nature medicine, 24(2), 194-202.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!