Monoclonal anti human fitc conjugated igg antibody

HNSCC cells were harvested and washed with PBS containing 3% bovine serum albumin. The cells were incubated with cetuximab (10 μg) for 1 h. The isotype control group was incubated with rituximab (10 μg) for 1 h. After the washes, monoclonal anti-human FITC-conjugated IgG antibody (Sigma-Aldrich, St. Louis, MO, USA) was added to cells and incubated for 1 h at 4°C. The cells were washed three times with PBS and analyzed using FACS Calibur (Becton Dickinson Biosciences, Franklin Lakes, NJ, USA) to measure the expression level of EGFR at the cell surface.

Breast cancer cells were harvested and washed with PBS containing 1% (w/v) bovine serum albumin (Sigma Aldrich, Saint Louis, MO, USA). Cells were incubated with 10 μg of pertuzumab (Perjeta^®, Roche, Basel, Switzerland) or isotype antibody, rituximab (Mabthera^®, Roche, Basel, Switzerland) for 1 h at 4 °C. After washing, 1 μL of monoclonal anti-human FITC-conjugated IgG antibody (Sigma Aldrich, F5016, Saint Louis, MO, USA) was added and incubated for 1 h at 4 °C. Stained cells were analyzed using FACS Calibur and CellQuest software (BD Biosciences Immunocytometry System, San Jose, CA, USA) to measure the HER2 expression level at the cell surface.