Plan apo vc 1.4 na

Confocal microscopy on myofibers was performed using a Nikon A1-Rs inverted laser scanning microscope with a 60× Plan Apo VC/1.4 NA oil-immersion objective. Transfected C2C12 images were acquired using DeltaVision Elite (GE Healthcare) and processed using SoftWoRx software (GE Healthcare).

MDCK cells were seeded as single cells on Matrigel-coated coverslips in complete medium containing 2% Matrigel (BD Biosciences). Supplementary Movie 1. Six hours after seeding, images were acquired (15 h, 5 min time interval) with the UltraVIEW VoX (Perkin Elmer) spinning disk confocal unit. Images were acquired with a 60X oil immersion objective (Nikon PLANApo VC 1.4 NA). Supplementary Movies 2 and 3. Six hours after seeding, images were acquired (15 h, 5 min time interval) using a Confocal Spinning Disk microscope (Olympus) equipped with IX83 inverted microscope provided with an IXON 897 Ultra camera (Andor), using a 60x UPlanSApo 1.35NA objective. The system is driven by the Olympus CellSens Dimension 1.18 software (Build 16686). The experiments were performed using an environmental microscope incubator (OKOLab) set to 37 °C and 5% CO2 perfusion.