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Pgbkt7 or pgadt7 vectors

Manufactured by Takara Bio
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The PGBKT7 or pGADT7 vectors are yeast two-hybrid system vectors used for the detection and analysis of protein-protein interactions. These vectors facilitate the expression of fusion proteins in yeast cells, allowing for the study of interactions between two proteins of interest. The core function of these vectors is to provide a platform for the investigation of protein-protein interactions, but a more detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Matchmaker gold y2h system user manual, by Takara Bio (2 mentions) X α gal, by Yeasen (1 mentions) Aureobasidin a, by Yeasen (1 mentions)

Spelling variants of this product

PGBKT7 (413 citations) PGADT7 (337 citations) PGADT7 vector (159 citations) PGBKT7 vector (151 citations) PGADT7/pGBKT7 (2 citations)

3 protocols using pgbkt7 or pgadt7 vectors

1

Yeast Two-Hybrid Assay for Protein-Protein Interactions

Cited 1 time
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The Y2H assays were performed as described54 (link) according to the Matchmaker Gold Y2H system user manual (Clontech). Constructs of the TREX-2 components were from Yuhai Cui’s lab24 (link). pGBKT7-NRPB1-CTD, pGBKT7-CDC5, pGBKT7-NOT2B and pGBKT7-SE were from Yijun Qi’s lab6 (link). Other full-length or truncated cDNAs were amplified and cloned into the pGBKT7 or pGADT7 vectors (Clontech), and verified by sequencing. For the Y2H assays, the pGBKT7 and pGADT7 plasmids with the genes to be tested were transformed into the Y2H Gold and Y187 yeast strains, respectively. The two yeast strains were mixed for mating, and the mating mixture was transferred to the DDO (lacking Trp and Leu) and QDO/X/A (lacking His, Ade, Trp, and Leu and containing X-α-gal and Aureobasidin A) media for selection of diploids and those with reporter gene expression, respectively.
Zhang B., You C., Zhang Y., Zeng L., Hu J., Zhao M, & Chen X. (2020). Linking Key Steps of MicroRNA Biogenesis by TREX-2 and the Nuclear Pore Complex in Arabidopsis. Nature plants, 6(8), 957-969.
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2

Yeast Two-Hybrid Assay for Protein-Protein Interactions

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The Y2H assays were performed as described54 (link) according to the Matchmaker Gold Y2H system user manual (Clontech). Constructs of the TREX-2 components were from Yuhai Cui’s lab24 (link). pGBKT7-NRPB1-CTD, pGBKT7-CDC5, pGBKT7-NOT2B and pGBKT7-SE were from Yijun Qi’s lab6 (link). Other full-length or truncated cDNAs were amplified and cloned into the pGBKT7 or pGADT7 vectors (Clontech), and verified by sequencing. For the Y2H assays, the pGBKT7 and pGADT7 plasmids with the genes to be tested were transformed into the Y2H Gold and Y187 yeast strains, respectively. The two yeast strains were mixed for mating, and the mating mixture was transferred to the DDO (lacking Trp and Leu) and QDO/X/A (lacking His, Ade, Trp, and Leu and containing X-α-gal and Aureobasidin A) media for selection of diploids and those with reporter gene expression, respectively.
Zhang B., You C., Zhang Y., Zeng L., Hu J., Zhao M, & Chen X. (2020). Linking Key Steps of MicroRNA Biogenesis by TREX-2 and the Nuclear Pore Complex in Arabidopsis. Nature plants, 6(8), 957-969.
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3

Rab7-RILP Interaction Analysis

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The interactions between Rab7 and RILP were analyzed by yeast two-hybrid assays. All the DNA sequences of Rab7 and RILP were inserted into pGBKT7 or pGADT7 vectors (TAKARA Clontech, China). After co-transfection of pGBKT7 and pGADT7, the yeast colonies were grown in DDO/X, SD/-Leu/-Trp double dropout media (Clontech, China) containing X-α-Gal (40 μg/mL, Yeasen, China) and QDO/X/A, SD/-Ade/-His/-Leu/-Trp quadruple dropout media (Clontech, China) containing X-α-Gal and aureobasidin A (200 ng/mL, Yeasen, China) agar plates. TDO/X/A, SD/-His/-Leu/-Trp triple dropout media containing X-α-Gal and aureobasidin A agar plates were also sometimes used. The biochemical incubator was cultured inverted at 30°C. The results (Figures S19–S24) were judged based on the state and color of the colonies (Positive, blue colonies on QDO/X/A or TDO/X/A plates; Negative, no colonies on DDO/X, QDO/X/A or TDO/X/A, or white colonies on QDO/X/A or TDO/X/A plates).
Cui G., Jiang Z., Chen Y., Li Y., Ai S., Sun R., Yi X, & Zhong G. (2023). Evolutional insights into the interaction between Rab7 and RILP in lysosome motility. iScience, 26(7), 107040.
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