Anti mouse igg horseradish hrp linked na931v

25HC and 27HC (Sigma) were dissolved in sterile ethanol at concentrations ranging from 2.75 mM to 3 mM. U18666A was purchased from Millipore (Darmstadt, Germany) and dissolved in sterile dimethyl sulfoxide (DMSO) to a concentration of 5 mM. Mouse monoclonal antibody (MAb) directed to human rotavirus VP6 (2B4) was purchased from Covalab (Villeurbanne, France). The rabbit polyclonal antibodies directed to VAP-A (H-40: sc-98890) and Rab 7 (H-50: sc-10767) were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). The rabbit polyclonal antibody directed to OSBP (C2C3) was purchased from GeneTex (Irvine, CA, USA). The rabbit polyclonal antibody to OSBP-N-terminal (ab192990) was purchased from Abcam (Cambridge, UK). The mouse monoclonal antibody directed to actin (MAB1501R) was purchased from Millipore. The secondary antibody peroxidase-conjugated AffiniPure F(ab’)2 Fragment Goat Anti-Mouse IgG (H+L) was purchased from Jackson ImmunoResearch Laboratories Inc. (West Grove, PA, USA). The secondary antibodies anti-mouse IgG horseradish (HRP)-linked (NA931V) and anti-rabbit IgG HRP-linked (NA934V) were purchased from GE Healthcare UK (Chalfont St Giles, UK). The secondary antibodies goat anti-rabbit IgG-FITC (sc-2012) and goat anti-mouse IgG-R (sc-2092) were purchased from Santa Cruz Biotechnology, Inc.