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Flex peroxidase blocking

Manufactured by Agilent Technologies
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Flex Peroxidase Blocking is a laboratory reagent used to inhibit endogenous peroxidase activity in tissue samples. It helps prevent non-specific binding and false-positive results in immunohistochemical and other enzymatic assays.

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Lab products found in correlation

Link 48, by Agilent Technologies (2 mentions) Flex dab sub chromo, by Agilent Technologies (2 mentions) Envision flex hrp kit, by Agilent Technologies (2 mentions) Flex hrp, by Agilent Technologies (2 mentions) Citrate buffer ph 6, by Agilent Technologies (2 mentions)

Close spelling variants of this product

EnVision FLEX Peroxidase-Blocking Reagent Dako EnVision FLEX Peroxidase-Blocking Reagent Peroxidase blocking

2 protocols using flex peroxidase blocking

1

Immunohistochemistry staining of ovarian cancer samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
The single chromogenic immunohistochemistry staining was performed on 4 μm formalin-fixed paraffin-embedded (FFPE) sections of ovarian cancer resections using the EnVision-Flex HRP kit on the Dako autostainer Link48. Briefly, slides were heated at 60°C for 1 hour, deparaffinized by immersing the slides in 3 consecutive xylene baths, followed by rehydration by immersing slides in 2 consecutive ethanol decreasing grade baths (100%, 95% and 70%) and in water bath. The antigen retrieval was performed in Citrate buffer (pH6, Dako) for 20 min at RT. Peroxidase blocking was performed for 5 min at RT (Flex Peroxidase Blocking, DAKO), followed by primary Ab incubation (CD8, γH2AX, pSTAT1, total STAT1) for 1 h at RT. After several washes, slides were incubation with the secondary Ab (Flex-HRP, DAKO) for 20 min at RT and Peroxidase were revealed with diaminobenzidine-peroxidase substrate (Flex DAB+Sub chromo, Dako) for 10 min at RT. After several washes, slides were counterstained with Flex-Hematoxylin, dehydrated by immersing slides in successive ethanol baths (70%, 95%, 100%) and xylene baths. The slides were then mounted with Shandon-mount medium and analyzed by microscopic observation and semiquantitative analysis by a qualified pathologist (PGF). All Abs are listed in the Key resource table.
Bruand M., Barras D., Mina M., Ghisoni E., Morotti M., Lanitis E., Fahr N., Desbuisson M., Grimm A., Zhang H., Chong C., Dagher J., Chee S., Tsianou T., Dorier J., Stevenson B.J., Iseli C., Ronet C., Bobisse S., Genolet R., Walton J., Bassani-Sternberg M., Kandalaft L.E., Ren B., McNeish I., Swisher E., Harari A., Delorenzi M., Ciriello G., Irving M., Rusakiewicz S., Foukas P.G., Martinon F., Dangaj Laniti D, & Coukos G. (2021). Cell-autonomous inflammation of BRCA1-deficient ovarian cancers drives both tumor-intrinsic immunoreactivity and immune resistance via STING. Cell reports, 36(3), 109412.
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2

Immunohistochemistry staining of ovarian cancer samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
The single chromogenic immunohistochemistry staining was performed on 4 μm formalin-fixed paraffin-embedded (FFPE) sections of ovarian cancer resections using the EnVision-Flex HRP kit on the Dako autostainer Link48. Briefly, slides were heated at 60°C for 1 hour, deparaffinized by immersing the slides in 3 consecutive xylene baths, followed by rehydration by immersing slides in 2 consecutive ethanol decreasing grade baths (100%, 95% and 70%) and in water bath. The antigen retrieval was performed in Citrate buffer (pH6, Dako) for 20 min at RT. Peroxidase blocking was performed for 5 min at RT (Flex Peroxidase Blocking, DAKO), followed by primary Ab incubation (CD8, γH2AX, pSTAT1, total STAT1) for 1 h at RT. After several washes, slides were incubation with the secondary Ab (Flex-HRP, DAKO) for 20 min at RT and Peroxidase were revealed with diaminobenzidine-peroxidase substrate (Flex DAB+Sub chromo, Dako) for 10 min at RT. After several washes, slides were counterstained with Flex-Hematoxylin, dehydrated by immersing slides in successive ethanol baths (70%, 95%, 100%) and xylene baths. The slides were then mounted with Shandon-mount medium and analyzed by microscopic observation and semiquantitative analysis by a qualified pathologist (PGF). All Abs are listed in the Key resource table.
Bruand M., Barras D., Mina M., Ghisoni E., Morotti M., Lanitis E., Fahr N., Desbuisson M., Grimm A., Zhang H., Chong C., Dagher J., Chee S., Tsianou T., Dorier J., Stevenson B.J., Iseli C., Ronet C., Bobisse S., Genolet R., Walton J., Bassani-Sternberg M., Kandalaft L.E., Ren B., McNeish I., Swisher E., Harari A., Delorenzi M., Ciriello G., Irving M., Rusakiewicz S., Foukas P.G., Martinon F., Dangaj Laniti D, & Coukos G. (2021). Cell-autonomous inflammation of BRCA1-deficient ovarian cancers drives both tumor-intrinsic immunoreactivity and immune resistance via STING. Cell reports, 36(3), 109412.
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