Antibodies against β actin

Heat-inactivated fetal bovine serum (FBS) and cell culture medium DMEM, DMEM/F12 were purchased from Gibco (Invitrogen China Limited, China). A real-time PCR (RT-PCR) kit was purchased from TransGen Biotech (Beijing, China). D-Luciferin was purchased from Biosynth International (Naperville, USA). Coelenterazine was purchased from NanoLight Technology (Pinetop, AZ, USA). Antibodies against p-BRCA1 (Ser1524), 53BP1, p-ATM (Ser1981), p-p53 (Ser15), p53, p-CHK1 (Ser317), p-Stat3 (Tyr705), Stat3 were purchased from Abcam (Cambridge, MA, USA). Antibodies against β-actin, β-tubulin, and GADPH were obtained from ZSGB-Bio (China).

Osthole (purity > 98%) was purchased from Meilunbio (Dalian, China). MG‐132 and Nec‐1 were purchased from MedChemExpress (Shanghai, China). NAC and JC‐1 were purchased from Beyotime (Shanghai, China). DMSO and 2′, 7′‐dichloro‐hydrofluorescein diacetate (DCFH‐DA) were purchased from Sigma‐Aldrich (St. Louis, MO, USA). Anti‐rabbit IgG, anti‐mouse IgG, and antibodies against β‐actin were purchased from ZSGB‐BIO (Beijing, China). The anti‐RIP1 IgG, anti‐caspase‐3 IgG, anti‐caspase‐7 IgG and anti‐caspase‐9 IgG were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti‐RIP3 IgG, anti‐MLKL IgG and anti‐caspase‐8 IgG were purchased from Abcam (Cambridge, MA, USA).

Osthole and cisplatin (DDP) were purchased from Push biotechnology Co., Ltd (purity ≥ 99%, Chengdu, China). Dicoumarol (DIC), Z-VAD-FMK, Z-DEVD-FMK, and 3-methyladenine (3-MA) were purchased from MedChem Express (Princeton, NJ, USA). N-Acetyl-L-cysteine (NAC) and JC-1 were purchased from Beyotime (Shanghai, China). Dimethyl sulfoxide (DMSO) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Anti-rabbit IgG, anti-mouse IgG, and antibodies against β-actin were purchased from ZSGB-BIO (Beijing, China). Anti-NQO1, anti-caspase-3, anti-cleaved caspase-3, anti-PARP-1, and anti-GSDME antibodies were purchased from Abcam (Cambridge, MA, USA). FITC Annexin V Apoptosis Detection Kit was purchased from BD (BD Pharmingen, CA, USA).