Menadione

Manufactured by MedChemExpress

Sourced in China

Menadione is a synthetic compound that functions as a vitamin K3 analogue. It is a white to pale yellow crystalline powder that is commonly used as a starting material in organic synthesis. Menadione's core function is to serve as a precursor for the production of various vitamin K-dependent compounds.

Automatically generated - may contain errors

Lab products found in correlation

Staurosporine, by MedChemExpress (1 mentions) Celltiter glo 2, by Promega (1 mentions) Varioskan lux multimode reader, by Thermo Fisher Scientific (1 mentions) Caspase glo 3 7, by Promega (1 mentions) Ros glo h2o2, by Promega (1 mentions) Prism 7, by GraphPad (1 mentions) Cck 8 assay, by Dojindo Laboratories (1 mentions)

Close spelling variants of this product

Menadione (HY-B0332)

2 protocols using menadione

Dose-dependent Cytotoxicity and Apoptosis Assay

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To measure cell viability after 72 h of doxorubicin (0.01-100 μM) treatment, CellTiter-Glo 2.0 (Promega) was used per manufacturer’s instructions. Luminescence was measured using a VarioSkan Lux Multi-Mode Reader (Thermo Scientific) with an integration time of 0.25 sec. 10 μM staurosporine (MedChemExpress) was used as a positive control. After 24 h of doxorubicin (0.01-100 μM) treatment, apoptosis and ROS was measured using Caspase 3/7-Glo and ROS-Glo H₂O₂ (both Promega) respectively according to manufacturer’s instructions with an integration time of 1 sec. 10 μM staurosporine and 50 μM menadione (both MedChemExpress) was used as a positive control, respectively. Data were analyzed using Prism 7.0 software (GraphPad) using standard dose-response guidelines.

Magdy T., Jiang Z., Jouni M., Fonoudi H., Lyra-Leite D., Jung G., Romero-Tejeda M., Kuo H.H., Fetterman K.A., Gharib M., Burmeister B.T., Zhao M., Sapkota Y., Ross C.J., Carleton B.C., Bernstein D, & Burridge P.W. (2021). RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy. Cell stem cell.

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Menadione-Induced Cell Proliferation Assay

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Cell proliferation was detected using a CCK-8 assay (Dojindo, Japan). Briefly, cells were seeded in 96-well plates (3000 cells/well), cultured in DMEM for 24 h and treated with 10 μM menadione (MedChemExpress) for 0, 12, 24, or 48 h. After incubation, the cells were treated with CCK-8 reagents according to the manufacturer’s instructions and absorbance was measured at 450 nm using a multi-mode reader (LD942, Beijing, China). All assays were performed in triplicate.

Wang M., Zhong B., Li M., Wang Y., Yang H, & Du K. (2021). Identification of potential core genes and pathways predicting pathogenesis in head and neck squamous cell carcinoma. Bioscience Reports, 41(5), BSR20204148.

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