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Darpp 32 sirna sc 35173

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DARPP-32 siRNA (sc-35173) is a double-stranded RNA molecule designed to target and silence the expression of the DARPP-32 gene. DARPP-32 is a protein phosphatase 1 regulator that plays a role in neuronal signaling pathways. This product is intended for use in RNA interference (RNAi) experiments to study the function of DARPP-32 in cellular systems.

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Lab products found in correlation

Pcdna3.1 mammalian expression plasmid, by Thermo Fisher Scientific (2 mentions) Calcium phosphate transfection kit, by Applied Biological Materials (1 mentions)

Close spelling variants of this product

SiRNAs (95 citations) SC-32 (2 citations)

2 protocols using darpp 32 sirna sc 35173

1

DARPP-32 Expression in Cell Lines

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The flag-tagged coding sequence of DARPP-32 was cloned in pcDNA3.1 mammalian expression plasmid (Invitrogen Life Technologies). AGS cells stably expressing DARPP-32 or pcDNA3.1 empty vector were generated as described previously.4 (link)5 (link) Flag-tagged DARPP-32 was cloned into the adenoviral (pACCMV) shuttle vector, and the adenovirus was generated by cotransfecting HEK-293 cells with the shuttle and (pJM17) backbone adenoviral plasmids using the Calcium Phosphate Transfection Kit (Applied Biological Materials, Richmond, British Columbia, USA). Lentivirus particles expressing DARPP-32 shRNA or control shRNA were produced by GeneCopoeia (Rockville, Maryland, USA) and then used to transduce MKN-45 cells. Control siRNA (universal negative control) was purchased from Sigma–Aldrich; DARPP-32 siRNA (sc-35173) was obtained from Santa Cruz Biotechnology.
Zhu S., Soutto M., Chen Z., Peng D., Romero-Gallo J., Krishna U.S., Belkhiri A., Washington M.K., Peek R, & El-Rifai W. (2016). Helicobacter pylori-induced cell death is counteracted by NF-κB-mediated transcription of DARPP-32. Gut, 66(5), 761-762.
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2

Cloning and Silencing of DARPP-32

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The FLAG-tagged coding sequence of DARPP-32 was cloned in pcDNA3.1 mammalian expression plasmid (Invitrogen). Control siRNA (universal negative control1) was purchased from Sigma-Aldrich (St. Louis, MO); DARPP-32 siRNA (sc-35173) was obtained from Santa Cruz Biotechnology (Santa Cruz).
Zhu S., Khalafi S., Chen Z., Poveda J., Peng D., Lu H., Soutto M., Que J., Garcia-Buitrago M., Zaika A, & El-Rifai W. (2020). Silencing of miR490–3p by H. pylori activates DARPP-32 and induces resistance to gefitinib. Cancer letters, 491, 87-96.
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