Human m csf 1

Manufactured by Thermo Fisher Scientific

Sourced in United States

Human M-CSF-1 is a recombinant human protein that functions as a colony-stimulating factor. It is used in cell culture applications to support the growth and differentiation of macrophages.

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Lab products found in correlation

Macrophage serum free medium, by Thermo Fisher Scientific (1 mentions) Lymphoprep, by Axis-Shield (1 mentions) Human ab serum, by Corning (1 mentions) Human gm csf, by Thermo Fisher Scientific (1 mentions) Interleukin 4 (il 4), by Thermo Fisher Scientific (1 mentions) Easysep monocyte enrichment kit, by STEMCELL (1 mentions)

2 protocols using human m csf 1

Differentiation of Human Monocytes into Primary Macrophages

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All experiments using human primary cells were approved under ethical permit number Dnr.2017/582. Human CD14⁺ monocytes were isolated from buffy coats, provided by the Region Skåne Blodcentralen, using density gradient centrifugation over LymphoPrep (Axis Shield, United Kingdom) followed by CD14+ magnetic-activated cell sorting (Miltenyi Biotec, Cologne, Germany). Isolated CD14⁺ monocytes were differentiated into human primary macrophages by culture for 7 days in Macrophage Serum-Free medium (Gibco, Waltham, MA, USA), supplemented with 50 ng/ml human M-CSF-1 (Peprotech, Rocky Hill, NJ, USA), 100 U/ml penicillin and 100 μg/ml streptomycin, prior to experimental procedures.

Bierschenk D., Papac-Milicevic N., Bresch I.P., Kovacic V., Bettoni S., Dziedzic M., Wetsel R.A., Eschenburg S., Binder C.J., Blom A.M, & King B.C. (2023). C4b-binding protein inhibits particulate- and crystalline-induced NLRP3 inflammasome activation. Frontiers in Immunology, 14, 1149822.

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Alveolar Macrophage Cytokine Induction by NETs

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Human alveolar macrophages were isolated from transplant BAL of donor
lungs approximately 2 hours after reperfusion by plastic adherence in DMEM
(Gibco) complete medium supplemented with 20% human AB serum (Corning) and 20
ng/ml human M-CSF-1 (Peprotech) for 48 hours before the addition of NET
fragments. Following stimulation with NET preparations for 24 hours supernatant
cytokine accumulation was measured for indicated cytokines with ProCarta Luminex
assays (ThermoFisher) in accordance with manufacturer’s recommendations.
To generate human monocyte-derived dendritic cells, peripheral blood monocytes
were isolated with the Easy Sep monocyte enrichment kit (Stemcell) from healthy
human volunteers. 5 × 10⁶ monocytes per ml were added in
6-well plates and cultured in DMEM complete medium supplemented with 10% AB
serum, human GM-CSF (100ng/ml; Peprotech) and IL-4 (50 ng/ml; Peprotech) for 6
days with refreshment of media every 2 days prior to use in experiments.

Scozzi D., Wang X., Liao F., Liu Z., Zhu J., Pugh K., Ibrahim M., Hsiao H.M., Miller M.J., Yizhan G., Mohanakumar T., Krupnick A.S., Kreisel D, & Gelman A.E. (2018). Neutrophil extracellular trap fragments stimulate innate immune responses that prevent lung transplant tolerance. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 19(4), 1011-1023.

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