Mouse monoclonal anti ha tag antibody

Transient transfections were performed with GeneJuice Transfection reagent (Novagen, Milan, Italy), according to the manufacturer’s instruction, or standard calcium phosphate method [65 ]. Chemicals were all purchased from AppliChem GmbH (Germany). The proteasome inhibitor MG-132 was purchased from Sigma-Aldrich (Milan, Italy). Mouse anti-6xHis tag antibody (GE Healthcare, Milan, Italy), anti-RIG-I (DDX58) polyclonal antibody (OriGene, Rockville, MD, USA), mouse anti-FLAG M2 monoclonal antibody (Agilent Technologies, Milan, Italy), mouse monoclonal anti-HA tag antibody, fluorescein (FITC)-labeled anti-mouse IgG and anti-mouse IgG HRP-conjugated were purchased from Sigma-Aldrich. Anti-rabbit IgG HRP-conjugated was supplied by Santa Cruz Biotechnology Inc. (Heidelberg,Germany) Ni-NTA sepharose was purchased from Novagen (Milan, Italy) and anti-Ub FK2 clone from Enzo Life Sciences (New York, USA).

Transient transfections were performed with GeneJuice^® Transfection reagent (Novagen, Milan, Italy), according to the manufacturer’s instructions, or standard calcium phosphate method. The proteasome inhibitor MG-132 and cycloheximide (CHX) were purchased from Sigma-Aldrich (Milan, Italy). Mouse anti-6×His tag antibody (GE Healthcare, Milan, Italy), mouse monoclonal anti-HA tag antibody, and HRP-conjugated anti-mouse IgG were purchased from Sigma-Aldrich. Ni-NTA sepharose was from Novagen (Milan, Italy).