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Mini protean tgxtm gels for western blotting

Manufactured by Bio-Rad
Sourced in United States

The Mini-Protean® TGXTM gels are precast polyacrylamide gels designed for western blotting applications. They provide a fast and convenient solution for protein separation and transfer.

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2 protocols using mini protean tgxtm gels for western blotting

1

Mitoprobe JC-1 Assay Protocol

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Mitoprobe JC-1 kit was purchased from Life Technologies Corporation (Carlsbad, CA); rabbit anti-Slc40al (#NBP1–21502), rabbit anti-Abcb6 (#NBP2–58327), mouse anti-Gstpl (#NB110–60512) and rabbit anti Gsta4 (#H00002941-D01P) were purchased from Novus Biologicals (Littleton, CO). Mini-Protean® TGXTM gels for western blotting were purchased from Bio-rad (Hercules, CA, USA). Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) secondary antibodies were from GE Healthcare (Piscataway, NJ); Alexa Fluor” 488 or 555 conjugated goat anti-rabbit or anti-mouse antibodies were obtained from Invitrogen (Carlsbad, CA). RNeasy Plus Universal Mini Kit (#73404) were purchased from RNAeasy plus mini kit (Qiagen Inc, Germantown, MD). Sterile culture wares were purchased from Fisher Scientific (Pittsburgh, PA, USA) while molecular biology grade reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-rad laboratories (Hercules, CA, USA). RNA Stabilization Reagent (RNA later, cat #76104).
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2

Sulforaphane Regulation of Nrf2 and NQO1

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L-Sulforaphane (SFN) was purchased from Sigma-Aldrich (St. Louis, MO). Rabbit anti-Nrf2 (#sc-722) and mouse anti-Nqo1 (#sc-376023) antibodies were from Santa Cruz Biotechnology (Dallas, TX); rabbit anti-Slc40a1 was from Novus Biologicals (Littleton, CO). Mouse anti-Glut1 (clone 5B12.3) from EMD Millipore (Billerica, MA); Mini-Protean® TGXTM gels for western blotting were purchased from Bio-rad (Hercules, CA, USA). Donkey anti-rabbit (#NA934) and sheep anti-mouse (#NA931) secondary antibodies were from GE Healthcare (Piscataway, NJ); Alexa Fluor® 488 or 555 conjugated goat anti-rabbit or anti-mouse antibodies were obtained from Invitrogen (Carlsbad, CA). Sterile culture wares were purchased from Fisher Scientific (Pittsburgh, PA, USA) while molecular biology grade reagents and chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA) or Bio-rad laboratories (Hercules, CA, USA).
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