Milli q academic a10 water purification system

Prednisone powder was purchased from Skyrun (Taizhou, China). Microcrystalline Cellulose (Comprecel^® M102 D+) was purchased from Mingtai chemicals (Taoyuan Hsien, Taiwan). Eudragit^® RL 30 D was donated by Rohm Pharma (Darmstadt, Germany). Sodium starch glycolate, talc, magnesium stearate, Tween^® 80 were donated by Aspen Pharmacare (Port Elizabeth, South Africa). Polyethylene Glycol (PEG) 400 was purchased from Merck chemicals (Johannesburg, South Africa). Hydrochlorothiazide was purchased from Skyrun (Taizhou, China). HPLC far UV grade acetonitrile (ROMIL-SpS^™) was purchased from Microsep (Port Elizabeth, South Africa). The HPLC grade water used for analyses was prepared using a Milli-Q^® Academic A10 water purification system (Millipore^®, Bedford, MA, USA), consisting of an Ionex^® ion-exchange cartridge and a quantum EX-ultrapore organex cartridge, which was fitted with a 0.22 μm Millipak^® 40 sterile filter (Millipore^®, Bedford, MA, USA).

Chitosan low molecular weight (CS), acrylic acid (AA), N,N methylene bisacrylamide (MBA), Mono-potassium phosphate (KH₂PO₄), and sodium phosphate dibasic (Na₂HPO₄) were purchased from Sigma-Aldrich (Darmstadt, Germany). Tenofovir disoproxil fumarate (TDF) was donated by Aspen Pharmacare (Durban, South Africa). Acrylamide (AAm), hydrogen chloride (HCl), and HPLC grade acetonitrile were purchased from Merck (Darmstadt, Germany). Ammonium persulfate and orthophosphoric acid were purchased from Saarchem (Wadeville, South Africa). Glacial acetic acid, sodium hydroxide (NaOH), magnesium chloride (MgCl₂), sodium chloride (NaCl), potassium chloride (KCl), methanol, and acetone were purchased from Minema (Roodepoort, South Africa). Almunium chloride (AlCl₃) was purchased from Fluka Chemika (Buchs, Switzerland). Ultra-pure water was HPLC grade 18 mega Ohm water, prepared using a Milli-Q academic A10 water purification system (Millipore^® Bedford, MA, USA).