Prasugrel metabolite r 138727

Adenosine receptor agonists were purchased from Sigma (St. Louis, MO, USA) (NECA (CAS № 35920-39-9)), and Cayman (Ann Arbor, MI, USA) (regadenoson (CAS № 313348-27-5)). LUF5835 (2-amino-6-(1H-imidazol-2-ylmethylsulfanyl)-4-(3-hydroxy-phenyl) pyridine-3,5dicarbonitrile) was synthesized at Laboratory of Molecular Virology and Biological Chemistry, Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland. Cangrelor (AR-C69931MX) was from Cayman Chemical (Ann Arbor, MI, USA). Prasugrel metabolite (R-138727) was obtained from BOC Sciences (Shirley, NY, USA). Calcein AM was obtained from Molecular Probes (Eugene, OR, USA). Antibodies anti-human CD61/PErCP, CD61/PE, CD62/PE, PAC-1/FITC, mouse IgG1/PE isotype control, mouse IgG1/FITC isotype control, Cellfix, buffered sodium citrate was purchased from Becton-Dickinson (San Diego, CA, USA). Phosphate buffered saline pH 7.4 (PBS) was obtained from Corning (New York, NY, USA). Dimethyl sulfoxide (DMSO), adenosine diphosphate (ADP), and bovine serum albumin (BSA) were obtained from Sigma (St. Louis, MO, USA). All other chemicals, unless otherwise stated, were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland).