At the age of 10 weeks, sitagliptin (sitagliptin phosphate monohydrate (Y0001812, Merck KGaA, Darmstadt, Germany)) eyedrops (10 mg/mL; n = 12) and vehicle (phosphate-buffered saline (PBS)) eyedrops (n = 12) were randomly administered twice per day directly onto the superior corneal surface of each eye of diabetic mice with the aid of a micropipette (5 µL). On day 15, animals (12 weeks of age) received one drop of sitagliptin or vehicle 1 h before euthanasia. Twelve non-diabetic mice, matched by age, were used as a control group.
Sitagliptin phosphate monohydrate
Manufactured by Merck Group
Sourced in Germany
Sitagliptin phosphate monohydrate is a chemical compound used in the production of pharmaceutical products. It is a white to off-white crystalline powder. The compound is soluble in water and in many organic solvents.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Hydrofluoric acid, by Merck Group (1 mentions)
Cell culture insert, by Thermo Fisher Scientific (1 mentions)
Pancreatin, by Merck Group (1 mentions)
Glutamine, by Thermo Fisher Scientific (1 mentions)
α linolenic acid, by Merck Group (1 mentions)
Glp 1 active elisa kit eglp 35k, by Merck Group (1 mentions)
Dpp4 inhibitor, by Merck Group (1 mentions)
D glucose, by Avantor (1 mentions)
Pepsin, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Lecithin, by Merck Group (1 mentions)
Carboxymethylcellulose sodium, by Merck Group (1 mentions)
4 protocols using sitagliptin phosphate monohydrate
1
Sitagliptin Eye Drops for Diabetic Mice
2
In Vitro GLP-1 Analysis Protocol
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Hydrofluoric acid (HF), α‐Linolenic acid, DPP4 inhibitor, DMEM, pancreatin, pepsin, lecithin, sodium taurocholate hydrate, DMSO, carboxymethylcellulose sodium (CMC), sitagliptin phosphate monohydrate, and GLP‐1 (active) ELISA kit (EGLP‐35K) for in vitro GLP‐1 analysis were purchased from Merck (Germany). Methanol, cell culture inserts, fetal bovine serum (FBS), horse serum, penicillin‐streptomycin, glutamine were acquired from Thermo Fisher Scientific Inc. (Denmark). D‐ (+)‐Glucose was obtained from VWR International Oy (Finland), and EDTA‐coated capillary (Minivette POCT) was bought from SARSTEDT Oy (Finland). For measuring plasma active GLP‐1 level of mice, active GLP‐1 ELISA kits (293‐79301) were purchased from FUJIFILM Wako Pure Chemical Co. (Japan). Silicon wafers were purchased from Siegert Wafer GmbH (Germany), while ethanol (EtOH) was purchased from Altia Oyj (Finland).
3
Topical sitagliptin eye drops for diabetic mice
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Db/db mice aged 10 weeks received a topical ocular administration of 10 mg/mL sitagliptin phosphate monohydrate (Y0001812, Merck KGaA, Darmstadt, Germany) or vehicle eye drops [phosphate buffered saline (PBS)] for 2 weeks twice per day. Eye drops were randomly administered with the aid of a micropipette (5 µL), onto the superior corneal surfaces of both eyes of diabetic mice. On day 15, 1 h before euthanasia, animals received an additional dose of each treatment. Db/+ mice matched by age were used as the control group.
4
Sitagliptin Eyedrops Affect Corneal Health
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Sitagliptin [sitagliptin phosphate monohydrate (Y0001812, Merck KGaA, Darmstadt, Germany) eyedrops (10 mg/mL; 5 µL; n = 12) or vehicle [phosphate buffered saline (PBS)] eyedrops (5 µL; n = 12) were randomly administered directly onto the superior corneal surface of each eye using a micropipette in 10-week-old mice. The treatment (sitagliptin or vehicle) was administered twice daily for 15 days. Non-diabetic mice (n = 12) matched by age were used as control group. On day 15, the drop of sitagliptin or vehicle was administered animals 1h before euthanasia.
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