The dried fruits, leaves and root barks of L. barbarum were purchased from Ningxia Province. Rutin, kukoamine B, chlorogenic acid, scopolin, solasonine and N-p-trans-coumaroyltyramine were purchased from Baoji Herbest Bio-Tech Co., Ltd. (Baoji, China). 5,6-Dihydrosolasonine were isolated from the fruit of L. barbarum and identified by HR-ESI-MS and NMR techniques. Purities of all compounds were above 96% by HPLC analysis. HPLC grade methanol, acetonitrile, and MS grade formic acid were purchased from Thermo Fisher Scientific (Pittsburgh, PA, USA). Other chemicals and solvents were of analytical reagent grade.
L02 cells (normal human hepatic cell line) were obtained from National Experimental Cell Resource Sharing Platform (Beijing, China). Gentamicin, fetal bovine serum (FBS) were acquired from Gibco (Invitrogen, Paisley, UK). Dimethyl sulphoxide (DMSO) was purchased from Panreac (Barcelona, Spain). Dulbecco’s modified Eagle’s medium (DMEM), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hydrogen peroxide (30% w/w), 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), ascorbic acid and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
L02 cells (normal human hepatic cell line) were obtained from National Experimental Cell Resource Sharing Platform (Beijing, China). Gentamicin, fetal bovine serum (FBS) were acquired from Gibco (Invitrogen, Paisley, UK). Dimethyl sulphoxide (DMSO) was purchased from Panreac (Barcelona, Spain). Dulbecco’s modified Eagle’s medium (DMEM), 2,2-diphenyl-1-picryl-hydrazyl (DPPH), hydrogen peroxide (30% w/w), 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), ascorbic acid and 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from Sigma-Aldrich (St. Louis, MO, USA).