Wako nefa c kit

Manufactured by Thermo Fisher Scientific

Sourced in France

The Wako-NEFA-C kit is a laboratory product designed for the quantitative determination of non-esterified fatty acids (NEFA) in various biological samples. It provides a colorimetric assay for the accurate measurement of NEFA levels.

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Lab products found in correlation

Cholesterol rtu kit, by bioMérieux (1 mentions) Triglycerides pap kit, by bioMérieux (1 mentions)

Close spelling variants of this product

Kit c

2 protocols using wako nefa c kit

Quantification of Lipid Metabolites

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Liver and muscle samples were homogenized in aqueous NaCl solution (9 g/L) and Triton X-100 (0.1%) and free fatty acids, triglycerides and total cholesterol levels were quantified on the tissue homogenate by enzymatic methods (Wako-NEFA-C kit, Oxoid, Dardilly, France; Cholesterol CHOD-PAP SOBIODA kit and triglycerides LQ SOBIODA kit, Sobioda 38330 Montbonnot-Saint-Martin, France) [21] .

Bonafos B., Cortés-Espinar A.J., Balas L., Pessemesse L., Lambert K., Benlebna M., Gaillet S., Pelletier F., Delobel P., Ávila-Román J., Abellán M.M., Bertrand-Gaday C., Durand T., Coudray C., Casas F, & Feillet-Coudray C. (2023). 9-PAHPA long term intake in DIO and db/db mice ameliorates insulin sensitivity but has few effects on obesity and associated metabolic disorders. The Journal of nutritional biochemistry, 112.

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Muscle Lipid Profiling Protocol

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Muscle samples were homogenised in NaCl (9 g/l) and Triton X-100 (0•1 %), and levels of FFA, TAG and total cholesterol were quantified on the muscle homogenate by enzymatic methods (Wako NEFA-C kit; Oxoid; Cholesterol RTU kit and triglycerides PAP kit; Biomérieux).

Coudray C., Fouret G., Lambert K., Ferreri C., Rieusset J., Blachnio-Zabielska A., Lecomte J., Ebabe Elle R., Badia E., Murphy M.P, & Feillet-Coudray C. (2016). A mitochondrial-targeted ubiquinone modulates muscle lipid profile and improves mitochondrial respiration in obesogenic diet-fed rats. The British journal of nutrition, 115(7).

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