Mouse anti ptbp1

Manufactured by Thermo Fisher Scientific

Mouse anti-PTBP1 is a primary antibody that specifically binds to the PTBP1 (Polypyrimidine Tract Binding Protein 1) protein. PTBP1 is a RNA-binding protein involved in various cellular processes, including pre-mRNA splicing, mRNA stability, and translation regulation.

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Lab products found in correlation

Rabbit anti phospho akt ser473, by Cell Signaling Technology (2 mentions) Rabbit anti pan akt, by Cell Signaling Technology (2 mentions) Ecl prime, by GE Healthcare (2 mentions) Mouse anti hsc70, by Santa Cruz Biotechnology (1 mentions) Rabbit anti grp78, by Abcam (1 mentions) Rabbit anti gapdh, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Anti-PTBP1 mouse monoclonal antibody Anti-PTBP1 PTBP1

2 protocols using mouse anti ptbp1

Western Blot Analysis of Intestinal Proteins

Cited 1 time

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Isolated small intestinal crypt cells were homogenized in the lysis buffer as previously described (39 (link)). Protein lysates were cleared by spinning the samples twice at 4°C. Subsequently, samples were separated on SDS-PAGE and analyzed by western blotting as previously described (39 (link)). Primary antibodies used are mouse anti-PTBP1 (Life Technologies, 324800), mouse anti-PTBP2 antibody (ABCAM, sc-376316), rabbit anti-Pan AKT (Cell Signaling, 4685), rabbit anti-Phospho-AKT (Ser473) (Cell Signaling, 4060), mouse anti-HSC70 (Santa Cruz Biotechnology, SC-7298), and rabbit anti-GRP78 (Abcam, ab32618). Membranes were incubated with HRP-linked secondary antibodies and developed using ECL prime (G&E Healthcare Life Sciences).

Chembazhi U.V., Tung W.S., Hwang H., Wang Y., Lalwani A., Nguyen K.L., Bangru S., Yee D., Chin K., Yang J., Kalsotra A, & Mei W. (2023). PTBP1 controls intestinal epithelial regeneration through post-transcriptional regulation of gene expression. Nucleic Acids Research, 51(5), 2397-2414.

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Western Blot Analysis of Intestinal Crypt Cells

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Isolated small intestinal crypt cells were homogenized in lysis buffer as described [34] . Protein lysates were cleared by spinning the samples twice at 4°C. Subsequently, samples were separated on SDS-PAGE and analyzed by western blotting as described [34] . Primary antibodies used are mouse anti-PTBP1 (Life Technologies, 324800), mouse anti-PTBP2 antibody (Santa Cruz, sc-376316), rabbit anti-Pan AKT (Cell Signaling, 4685), rabbit anti-Phospho-AKT (Ser473) (Cell Signaling, 4060), rabbit anti-GAPDH (Santa Cruz Biotechnology, SC-32233), and rabbit anti-GRP (Abcam, ab32618). Membranes were incubated with HRP-linked secondary antibodies and developed using ECL prime (G&E Healthcare Life Sciences).

Tung W., Chembazhi U.V., Yang J., Nguyen K.L., Lalwani A., Bangru S., Yee D., Chin K., Kalsotra A., & Mei W. (2021). PTBP1/HNRNP I controls intestinal epithelial cell regeneration by maintaining stem cell survival and stemness.

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