The cells were harvested and re-suspended in 0.5 ml binding buffer, then incubated with Annexin-V fluorescein isothiocyanate/PI dual stain (BD Biosciences) for 15 min and finally determined by flow cytometry.
Annexin 5 fluorescein isothiocyanate pi dual stain
Manufactured by BD
Sourced in United States
Annexin-V fluorescein isothiocyanate/PI dual stain is a laboratory reagent used to detect apoptosis in cells. It contains Annexin-V, a protein that binds to phosphatidylserine, and propidium iodide (PI), a DNA-binding dye. This combination allows for the identification of cells undergoing early and late stages of apoptosis.
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2 protocols using annexin 5 fluorescein isothiocyanate pi dual stain
1
Apoptosis Detection by Flow Cytometry
2
Apoptosis Quantification by Flow Cytometry
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Cells were harvested, resuspended in 0.5 ml binding buffer, and incubated with Annexin V-fluorescein isothiocyanate/PI dual stain (BD Biosciences, San Jose, CA, USA) for 20 min and determined by flow cytometry. The assays were repeated three times. Cells negative for both PI and Annexin V were considered viable cells. PI-negative and Annexin V-positive cells were considered early apoptotic cells. PI-positive and Annexin V-positive cells were considered dead cells.
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