Saline reservoir

IOP was elevated in the right eye of forty-five Sprague-Dawley rats as described^{63 (link)}. Briefly, ketamine: xylazine anaethesia (75:10 mg/kg) was administered intraperitoneally and eyes received one drop each of proparacaine and tropicamide to induce analgesia and pupil dilation, respectively. A 30 g needle, connected to a saline reservoir (0.9% sodium chloride; Baxter International Inc., Deerfield, IL), was inserted into the anterior chamber parallel to the iris and secured in place. The reservoir was elevated to increase the IOP to 130 mmHg for 60 min. IOP was measured using a pressure transducer (BIOPAC Systems, Goleta, CA, USA) and a handheld tonometer (Icare TONOLAB, Finland). After 60 min, the reservoir was lowered, the needle removed, and a drop of gentamicin applied (Akorn, Lake Forest, IL).

Intraocular pressure (IOP) elevation was achieved via a vitreous chamber cannula placed in one randomly chosen eye. As previous^{29 (link)}, the cannula consisted of a 27 G needle (Fig. S1) connected to a saline reservoir (60 mL, Baxter International Inc. Toongabbie, NSW, Australia) via polyethylene tubing (0.8 mm outer, 0.4 mm inner diameter, Unomedical, Sydney, NSW, Australia). IOP was controlled by placing the reservoir to heights pre-calibrated against a manometer (Livingstone, Sydney, NSW, Australia).