The surface phenotype of untreated and A83-01-treated eMSC was assessed by flow cytometry for three MSC markers [SUSD2, CD140b (Platelet-derived growth factor receptor β) and CD90 (Thy-1)] as described previously (Gurung et al., 2015 ). Cells were incubated for 1 h with 1:20 primary or matched isotype control antibodies in PBS containing 2% FCS at 4°C in dark. The primary antibodies were APC-conjugated SUSD2 (Biolegend), PE-conjugated CD140b (R&D Systems, United States) and APC-conjugated CD90 (BD Pharmingen, United States). Cells were washed with 2% FCS/PBS and fixed with 1:1 4% paraformaldehyde in 2% FCS/PBS. eMSC were analyzed using BD FACSCanto II (BD) (10,000 events/sample) and FlowJo v.10 software.
Pe conjugated cd140b
Manufactured by R&D Systems
Sourced in United States
PE-conjugated CD140b is a fluorochrome-labeled antibody that binds to the CD140b (PDGFR-β) cell surface receptor. CD140b is a tyrosine kinase receptor that plays a role in various cellular processes. The PE (phycoerythrin) fluorescent label allows for the detection and analysis of CD140b-expressing cells using flow cytometry or other fluorescence-based techniques.
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2 protocols using pe conjugated cd140b
1
Phenotypic Characterization of eMSCs
2
Evaluating eMSC Surface Phenotype
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The surface phenotype of untreated and A83-01-treated eMSC was assessed by flow cytometry for three MSC markers [SUSD2, CD140b (Platelet-derived growth factor receptor β) and CD90 (Thy-1)] as described previously. 23 Cells were incubated for 1 h with 1:20 primary or matched isotype control antibodies in PBS containing 2% FCS at 4°C in dark. The primary antibodies were APC-conjugated SUSD2 (Biolegend), PE-conjugated CD140b (R&D Systems, USA) and APC-conjugated CD90 (BD Pharmingen, USA). Cells were washed with PBS/2% FCS and fixed with 4% paraformaldehyde in PBS/2% FCS. eMSC were analysed using BD FACSCanto II (BD) (10,000 events /sample) and FlowJo v.10 software.
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