The relative levels of Rap1, RhoA, Ve-cadherin, MLC expression and MLC phosphorylation in individual groups of cells were quanti ed by Western blot. Brie y, the different groups of cells were harvested and lyzed in lysis buffer, followed by centrifuged. After quanti cation of protein concentrations, the cell lysates (50 µg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on 10% gels and transferred onto polyvinylidene di uoride (PVDF) membranes. After being blocked, the membranes were probed with primary antibodies including anti-Rap1 (Abcam, ab181858, UK), anti-RhoA, (Proteintech, 10749-1-AP, USA), anti-MLC (Proteintech, 10906-1-AP), anti-p-MLC (Cell Singnaling Technology, 3671, USA), anti-Ve-cadherin (Cell Singnaling Technology, 2500) and anti-GAPDH (Yeasen Biotech, 33106ES60, China). After being washed, the bound antibodies were detected with HRPconjugated second antibodies and visualized with enhanced chemiluminescent reagents. The relative levels of each target to the control protein were quanti ed by densitometric analysis using Image-Pro Plus software (Media Cybernetics).
Anti mlc
Manufactured by Proteintech
Sourced in United States
Anti-MLC is a laboratory equipment product used for the detection and quantification of myosin light chain (MLC) proteins. It provides a reliable and efficient method for analyzing MLC levels in various biological samples.
Automatically generated - may contain errors
2 protocols using anti mlc
1
Quantifying Cell Signaling Proteins
2
Quantification of Cell Signaling Proteins
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The relative levels of Rap1, RhoA, Ve-cadherin, MLC expression and MLC phosphorylation in individual groups of cells were quantified by Western blot. Briefly, the different groups of cells were harvested and lyzed in lysis buffer, followed by centrifuged. After quantification of protein concentrations, the cell lysates (50 µg/lane) were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on 10% gels and transferred onto polyvinylidene difluoride (PVDF) membranes. After being blocked, the membranes were probed with primary antibodies including anti-Rap1 (Abcam, ab181858, UK), anti-RhoA, (Proteintech, 10749-1-AP, USA), anti-MLC (Proteintech, 10906-1-AP), anti-p-MLC (Cell Singnaling Technology, 3671, USA), anti-Vecadherin (Cell Singnaling Technology, 2500) and anti-GAPDH (Yeasen Biotech, 33106ES60, China). After being washed, the bound antibodies were detected with HRP-conjugated second antibodies and visualized with enhanced chemiluminescent reagents. The relative levels of each target to the control protein were quantified by densitometric analysis using Image-Pro Plus software (Media Cybernetics).
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