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Vhx 700f digital microscope

Manufactured by Keyence
Sourced in Japan

The VHX-700F Digital Microscope is a versatile laboratory equipment designed for high-resolution imaging and analysis. It features a powerful camera and advanced optics to capture detailed images and measurements of small samples. The core function of the VHX-700F is to provide users with a comprehensive visual inspection and documentation tool for a wide range of applications.

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12 protocols using vhx 700f digital microscope

1

Wax-Printed Paper-Based Microfluidics

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All designs within this study were printed on Whatman grade 1 cellulose chromatography paper (0.18 mm thickness) using a Colorqube 8580 Solid Ink color printer (Xerox, Norwalk, CT, USA). The wax in this system consists of multiple colors (cyan, magenta, yellow, black). The viability testing of all µPADs or line strokes were conducted with solutions of blue or red food dye (Kroger, Cincinnati, OH, USA) (1 drop/10 mL water). µPADs were heated on a Hotronix Auto-Open Clam heat press (Stahl’s, Sterling Heights, MI, USA). Measurements of wax spreading and permeation were taken with a Keyence VHX-700F digital microscope (Keyence, Itasca, IL, USA). A heat press was chosen as the source of heating, due to its reproducible time and temperature settings, as well as its ability to heat sheets of paper evenly on both sides.
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2

Characterization of Microneedle Arrays using SEM and Digital Microscopy

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Printed MN arrays were analysed using Scanning Electron Microscopy (SEM), using Hitachi TM3030 equipment (Tokyo, Japan). The arrays were viewed in the Energy Dispersive X-Ray (EDX) condition. The MN arrays were mounted onto the sample holder with double-sided carbon tape, placed into the SEM chamber, and analysed under vacuum. Measurements of base diameter and tip size were recorded. MN heights were recorded using optical light microscopy Leica EZ4D (Leica Microsystems, Milton Keynes, UK). A Keyence VHX-700F Digital Microscope (Keyence, Osaka, Japan) was also used to visualise the MNs, allowing for 3D reconstruction of the MN array structures.
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3

Characterization of Curcumin-Loaded Microneedle Arrays

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The shapes of CU-MN arrays were examined by using a Keyence VHX-700F Digital Microscope (Keyence, Osaka, Japan). A TA.XT-Plus Texture Analyser (Stable Microsystems, Haslemere, UK) was used in compression mode to assess the compression and insertion properties of CU-MN. Heights of CU-MN before compression were first determined using the digital microscope. The curcumin CU-MN arrays were then attached using double-sided adhesive tape to the movable cylindrical probe of the Texture Analyser and pressed by the test station against a flat aluminium block at a rate of 0.5 mm/s for 30 s at a force of 32 N (0.0.264 N/needle) [18 (link)]. Pretest and post-test speeds were set at 1 mm/s, and the trigger force was set at 0.049 N. CU-MN heights were measured again, post-compression, using the digital microscope.
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4

Imaging Nanoparticles in Microneedle Arrays

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The surface morphology and shape of CL‐NS‐loaded DMN arrays were examined by using a Keyence VHX‐700F Digital Microscope (Keyence, Osaka, Japan) and a TM3030 benchtop scanning electron microscope (SEM) (Hitachi, Krefeld, Germany). The latter was used in low vacuum mode at a voltage of 15 kV. In the case of NS particles, visualization inside the DMN arrays was achieved by SEM with a Quanta FEG 250 (FEI, Hillsboro, OR, USA) at an acceleration voltage of 10–20 kV under high chamber pressure (8 × 10−5 mbar) with standard SEM carbon tape as background.
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5

Surface Morphology of DFS-NS-DMNs

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The surface morphology and shape of DFS-NS-DMNs were examined using a Keyence VHX-700F Digital Microscope (Keyence, Osaka, Japan) and a TM3030 benchtop scanning electron microscope (SEM) (Hitachi, Krefeld, Germany). The latter was used in low vacuum mode at a voltage of 15 kV.
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6

Visualizing AmB-NE-DMN Surface Morphology

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The surface morphology and shape of AmB-NE-DMN were examined by using optical and scanning electron microscopy. A Keyence VHX-700F Digital Microscope (Keyence, Osaka, Japan) and TM3030 benchtop scanning electron microscope (SEM) (Hitachi, Krefeld, Germany) were used for evaluation. The SEM was used in low vacuum mode at a voltage of 15 kV [38 (link)].
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7

Multimodal Imaging Protocol for Biological Samples

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Microscopy images were obtained using a Leica EZ4 D digital microscope (Leica, Wetzlar, Germany) and a Keyence VHX-700F Digital Microscope equipped with a VH-Z20R lens (Keyence, Osaka, Japan). OCT images were recorder using an EX1301 OCT Microscope (Michelson Diagnostics Ltd., Kent, UK) and analysed using the imaging software ImageJ® (National Institutes of Health, Bethesda, USA).
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8

Characterization of Microneedle Array Properties

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Formed MN arrays were visualised using a Keyence VHX-700F Digital Microscope equipped with a VH-Z20R lens (Keyence, Osaka, Japan), which allows automatic sizing of multiple MN, facilitating quality control in terms of efficiency and reproducibility of MN formation within and between individual arrays. Additionally needle dimensions where evaluated using an Aigo Digital Viewer GE-5 (Aigo, Beijing, China). MN arrays were also subjected to compression tests in order to ascertain their mechanical strength. Mechanical properties were evaluated using a TA-XT2 Texture Analyser (Stable Microsystems, Haslemere, UK) in compression mode, as described previously [16] . MN arrays were visualised before and after application of the compression load using a desktop light microscope (GXMGE-5 digital microscope, Laboratory Analysis Ltd., Devon, UK).
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9

Characterization of Drug-Loaded DMN Patches

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Methylene blue (MB, 2% w/w) and fluorescein sodium (Flu—Na, 20% w/w), as a water-soluble blue (absorption wavelength of 664 nm) and red dye (absorption wavelength of 450 nm) respectively, were mixed into the PL (20% w/w) solutions and used as a model low molecular weight drugs. BSA-FITC (20% w/w), as a high molecular weight model protein with an excitation/emission wavelength of 480/520 nm, was dissolved in the PL solutions as well. Another model biomolecule, insulin (20% w/w), was dissolved in a 0.1 M HCl solution and then added to the PL solutions. These model drugs loaded PL DMN were prepared as per the described process in Section 2.4 and Fig. 1. The morphology of drug-loaded DMN patches was investigated using a Keyence VHX700F Digital Microscope (Keyence, Osaka, Japan). In addition, scanning electron microscopy (TM3030 benchtop SEM, Hitachi, Krefeld, Germany) was used for high-resolution imaging. The instrument was operated at 15 kV and images were captured at magnifications between 40 and 250×.
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10

New Marsdenia yarlungzangboensis Species

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Vouchers of Marsdeniayarlungzangboensis were collected from Motuo County, Xizang of China. The photographs and phenology data were obtained during the field expeditions.
Morphological observations and measurements of the new species were carried out based on living plants and dry specimens. The morphology of opened corolla, opened calyx, gynostegium and staminal corona, pistil, pollinarium were observed by using a Keyence VHX-700F Digital Microscope (Keyence, Osaka, Japan) and based on dry specimens. All morphological characters are described according to the terminology presented by Li et al. (1995) .
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