Sup b15

Manufactured by American Type Culture Collection

Sourced in United States, Germany

The SUP-B15 is a specialized laboratory equipment used for cell culture applications. It is designed to provide a controlled environment for the growth and maintenance of cell lines. The core function of the SUP-B15 is to regulate temperature, humidity, and gas composition to support the optimal conditions for cell proliferation and survival.

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Lab products found in correlation

53 protocols using sup b15

Comparative Analysis of Leukemia and Healthy B-cells

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The acute lymphoblastic leukemia cell line SUP-B15 (ATCC, CRL-1929) and healthy B-lymphocyte cell line NCI-BL 2171 (ATCC, CRL-5969) were commercially obtained. The SUP-B15 and NCI-BL 2171 cells were cultured in IMDM (Gibco, Cat. No: 12440053) and RPMI 1640 (Gibco, Cat. No: 11875085) mediums supplemented with 2 mM L-glutamine, 1% penicillin-streptomycin, and 10% FBS, respectively, in a cell culture incubator (Thermo Electron Corporation's Class 100) at 37°C, 95% humidity, and 5% CO₂. Light (Olympus-CH30) and inverted (Olympus-CKX41) microscopy were used to observe the viability and proliferation of the cells. The cell maintenance and all experiments were performed in an ultraviolet-sterilized laminar airflow cabinet (ESCO Class II, Biological Safety Cabinets).

Durmaz B., Bagca B.G., Cogulu O., Susluer S.Y., Alpay A., Aksoylar S, & Gunduz C. (2021). Antileukemic Effects of Anti-miR-146a, Anti-miR-155, Anti-miR-181a, and Prednisolone on Childhood Acute Lymphoblastic Leukemia. BioMed Research International, 2021, 3207328.

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Culturing Leukemia Cell Lines

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First time diagnosed leukemia cell lines: JM1 (CRL-10,423™, ATCC), SUP-B15 (CRL-1929 ™, ATCC), NALM-6 (CRL-3273™, ATCC). Relapsed leukemia cell lines: REH (ACC-2, DSMZ), and NALM-16 (ACC-680, DSMZ). JM1 and SUP-B15 cells were expanded and cultured in IMDM (Iscove's Modified Dulbecco's Medium) supplemented with 0.05 mM β-mercaptoethanol, 10% FBS, and 1 × Penicillin-Streptomycin. SUP-B15 cells were cultured in IMDM (Iscove's Modified Dulbecco's Medium), supplemented with 1.5 g/L sodium bicarbonate, 0.05 mM β-mercaptoethanol, 20% fetal bovine serum (not heat-inactivated), and 1 × Penicillin-Streptomycin. NALM-16, REH, NALM-6 cells were cultured in RPMI-1640 supplemented with 10% FBS, and 1 × Penicillin-Streptomycin. All were incubated in a humidified incubator at 37 °C with 5% CO2.

Haque S, & Vaiselbuh S.R. (2020). Vincristine and prednisone regulates cellular and exosomal miR-181a expression differently within the first time diagnosed and the relapsed leukemia B cells. Leukemia Research Reports, 14, 100221.

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Cell Culture Conditions for Cell Lines

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HUVEC (C2519A, Lonza) and human fibroblast cells (CC-2512, Lonza) were maintained in EGM-2 (CC-3162, Lonza) and FGM-2 (CC-3132, Lonza), respectively, prior to use in microfluidics experiments. Human B-ALL cell lines, SUP-B15 (ATCC, CRL-1929) and REH (ATCC, CRL-8286), were maintained in RPMI 1640 (Corning) supplemented with 20% Fetal Bovine Serum (FBS) (Sigma Aldrich) and 2-Mercaptoethanol (2-ME, 55nM) (Thermo Fisher Scientific). Retrovirus was generated using transfection of the Platinum-E cell line (Cell Biolabs, RV-101).

Witkowski M.T., Dolgalev I., Evensen N.A., Ma C., Chambers T., Roberts K.G., Sreeram S., Dai Y., Tikhonova A.N., Lasry A., Qu C., Pei D., Cheng C., Robbins G.A., Pierro J., Selvaraj S., Mezzano V., Daves M., Lupo P.J., Scheurer M.E., Loomis C.A., Mullighan C.G., Chen W., Rabin K.R., Tsirigos A., Carroll W.L, & Aifantis I. (2020). Extensive Remodeling of the Immune Microenvironment in B-cell Acute Lymphoblastic Leukemia. Cancer cell, 37(6), 867-882.e12.

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Cell Culture Protocols for Hematological Malignancies

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TOM1 (DSMZ ACC#578), SUPB15 (ATCC #CRL-1929), and JM1 (ATCC #CRL-10423) were purchased and maintained in RPMI 1640 supplemented with 10 % FBS, 0.05 mM β-mercaptoethanol and 1x streptomycin/penicillin antibiotics. REH (ATCC #CRL-8286), NALM1 (ATCC #CRL-1567), NALM6 (DSMZ ACC #128), BV173 (DSMZ ACC#20), RS4 (ATCC #CRL-1873) and SD1 (DSMZ ACC#366) were purchased and maintained in RPMI 1640 supplemented with 10 % FBS and 1x streptomycin/penicillin antibiotics. Primary CD3+ T cells, peripheral blood mononuclear cells (PBMC) and bone marrow mononuclear cells (BMMC) were purchased from AllCells (Allcells.com) and maintained in Lymphocyte Growth Medium-3 (Lonza, Cat No: CC-3211) containing 10% FBS and 1x streptomycin/penicillin. Human osteoblasts (HOB) were purchased from PromoCell (Cat No: C-12720, Heidelberg, Germany) and cultured according to the vendor’s recommendations. De-identified primary BMSC were provided by the WVU Cancer Institute Biospecimen Processing Core and the WVU Department of Pathology Tissue Bank. ALL cell lines were authenticated by short tandem repeat (STR) analysis (University of Arizona Genetics Core, Tucson, AZ) and maintained in 6 % CO₂ in normoxia at 37 °C. Pyrvinium pamoate (PP) was purchased from Sigma Aldrich (Cat #P-0027) and stored at −80 °C as a 10 mM stock.

Nair R.R., Piktel D., Hathaway Q.A., Rellick S.L., Thomas P., Saralkar P., Martin K.H., Geldenhuys W.J., Hollander J.M, & Gibson L.F. (2020). Pyrvinium pamoate use in a B-cell acute lymphoblastic leukemia model of the bone tumor microenvironment. Pharmaceutical research, 37(3), 43.

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Authenticated Leukemia and Lymphoma Cell Lines

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Established leukemia and lymphoma cell lines KCL-22 (CML; DSMZ no. ACC 519), K562 (CML; DSMZ no. ACC 10), SUP-B15 (BCR-ABL1+ B cell precursor leukemia; DSMZ no. ACC 389), Z-138 (Mantle cell lymphoma; ATCC no. CRL-3001), CML-T1 (CML; DSMZ no. ACC 7), JURKAT (T cell leukemia; DSMZ no. ACC 282), MAVER-1 (B cell lymphoma; DSMZ no. ACC 717), RAMOS (Burkitt lymphoma; DSMZ no. ACC 603) were obtained from publicly accessible biological resource centers (Leibniz Institute–Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH/DSMZ, Braunschweig, Germany; ATCC, Manassas, VA, USA). No cell lines used in this work are listed in the database of commonly misidentified cell lines. Cells were authenticated by providers and tested for Mycoplasma contamination using MycoAlert PLUS detection kit (LT07-705; Lonza Group AG, Basel, Switzerland). The latest testing for Mycoplasma contamination was up to 1 month after cells thawing. Cell lines were handled and cultivated in appropriate medium according the recommendations of the supplier. The cells were used in experiment within 2 months after thawing.

Jaruskova M., Curik N., Hercog R., Polivkova V., Motlova E., Benes V., Klamova H., Pecherkova P., Belohlavkova P., Vrbacky F, & Machova Polakova K. (2017). Genotypes of SLC22A4 and SLC22A5 regulatory loci are predictive of the response of chronic myeloid leukemia patients to imatinib treatment. Journal of Experimental & Clinical Cancer Research : CR, 36, 55.

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Pre-B Cell Acute Lymphoblastic Leukemia Cell Lines

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Generation and propagation of pre-B p190 mouse cells have been previously described [27 (link)]. Nalm-6 and Blin-1 cell lines were kindly provided by Dr. David Rawlings (University of Washington), and the RS11;4, SEM, and 697 cell lines by Dr. Anthony Letai (Harvard Med. School). The Ph+ cell lines SUP-B15, Tom-1, BV-173 and non-Ph lines REH and Kasumi-2 were purchased from ATCC and DSMZ. Except for the p190 cells, all described cells represent human pre-B cell acute lymphoblastic leukemia (B-ALL). All cells were maintained at 37°C in 5% CO2, and RPMI1640 supplemented with 1% L-glutamine, 1% Pen/Strep, 0.5% HEPES, 0.1% beta-mercaptoethanol and 10% FBS.

Beagle B.R., Nguyen D.M., Mallya S., Tang S.S., Lu M., Zeng Z., Konopleva M., Vo T.T, & Fruman D.A. (2014). mTOR kinase inhibitors synergize with histone deacetylase inhibitors to kill B-cell acute lymphoblastic leukemia cells. Oncotarget, 6(4), 2088-2100.

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Characterization of B-ALL Cell Lines

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NALM-6 (B-ALL), RS4;11 (KMT2A-rearranged B-ALL) and SUP-B15 (Ph-like B-ALL) were purchased from ATCC. NALM-6 and RS4;11 were cultured in RPMI 1640 (Corning) supplemented with 10% fetal bovine serum (FBS) (GeminiBio), 2 mmol/L L-glutamine (Gibco), and 100 mg/mL streptomycin/100 U/mL penicillin (Lonza). SUP-B15 cells were cultured in McCoy 5A (modified) media (Gibco) supplemented with 20% FBS, 100 mg/mL streptomycin and 100 U/mL penicillin. RS4;11 cells were transduced to express the luminescent reporter luciferase using pGL4.51[luc2/CMV/Neo] vector (Promega). Cell authentication was performed using short tandem repeat analysis (Idexx BioAnalytics, Westbrook, ME) and per ATCC guidelines using morphology, growth curves, and Mycoplasma testing within 6 months of use (InVivoGen). Cell lines were maintained in culture at 37°C in 5% CO₂.

Rinella S.P., Bell H.C., Turicek D.P., Shi L., Hoang N.M., Rui L., Hess N.J, & Capitini C.M. (2023). Combinatorial fedratinib and venetoclax treatment is effective on human B cell acute lymphoblastic leukemia with high Flt3 expression. bioRxiv.

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Maintenance of Ph+ ALL Cell Lines and Primary Samples

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BV173 (CML-lymphoid blast crisis cell line) were kindly provided by Dr N. Donato, (NIH), SUP-B15 (Ph+ ALL cell line) were purchased from ATCC, Z181 (Ph+ ALL cell line) were kindly provided by Dr. Z. Estrov, (M.D. Anderson Cancer Center, Houston, TX). TKI-resistant BV173 cells were generated by step-wise selection in the presence of increasing concentrations of imatinib, which induced the outgrowth of cells with the BCR-ABL1 T315I mutation. Experiments were performed on cell lines cultured for less than thirty passages. Mycoplasma was tested monthly following an established procedure (30 (link)). Cell lines were routinely authenticated by monitoring B-cell markers and BCR-ABL1 isoform expression. Cell lines were cultured in Iscove’s Medium (Gibco) supplemented with 10% fetal bovine serum, 100 U/mL penicillin–streptomycin and 2 mM L-glutamine at 37 °C. Primary human Ph+ ALL cells were maintained in SFEM (Stem Cell Technology) supplemented with SCF (40 ng/mL), Flt3L (30 ng/mL), IL-3 (10 ng/mL), IL-6 (10ng/mL) and IL-7 (10 ng/mL) (PeproTech). Information on primary Ph+ ALL samples used in this study is shown in Supplementary Table S1.

De Dominici M., Porazzi P., Soliera A.R., Mariani S.A., Addya S., Fortina P., Peterson L.F., Spinelli O., Rambaldi A., Martinelli G., Ferrari A., Iacobucci I, & Calabretta B. (2017). Targeting CDK6 and BCL2 exploits the “MYB addiction” of Ph+ acute lymphoblastic leukemia. Cancer research, 78(4), 1097-1109.

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Cultivation of Mouse and Human Cell Lines

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The spermatogonia
GC-1 cell line (ATCC CRL-2053)^{55 (link)} derived
from BALB/c mouse testes and the human B-lymphoblastic leukemia cell
line SUP-B15 (ATCC CRL-1929)^{56 (link)} were obtained
from the American Type Cell Culture Collection (ATCC). The GC-1 cell
line was cultured in Dulbecco’s modified Eagle’s medium
(DMEM; Sigma-Aldrich), while the SUP-B15 cell line was grown in RPMI-1640
complete medium (Sigma-Aldrich) supplemented with 10% fetal bovine
serum (Gibco, Dublin, Ireland) and 1% penicillin–streptomycin
(Sigma-Aldrich). Cells were incubated in 5% CO₂ at 37 °C.

Sopko B., Tejral G., Bitti G., Abate M., Medvedikova M., Hajduch M., Chloupek J., Fajmonova J., Skoric M., Amler E, & Erban T. (2021). Glyphosate Interaction with eEF1α1 Indicates Altered Protein Synthesis: Evidence for Reduced Spermatogenesis and Cytostatic Effect. ACS Omega, 6(23), 14848-14857.

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Culturing and Tracking Leukemia Cells

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The human bone marrow stromal cell line, HS-5, and the Philadelphia chromosome positive B cell acute lymphoblastic leukemia cell line, SUP-B15, were purchased from ATCC. HS-5 was cultured in Dulbecco’s Modified Eagle’s Medium plus 10% fetal bovine serum. SUP-B15 was maintained in Iscove’s Modified Dulbecco’s Medium with 4 mM l-glutamine, 1.5 g/l sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 20% fetal bovine serum, and 50 U/ml penicillin/streptomycin. In order to be tracked in vivo in animals, SUP-B15 cells were infected with lentivirus-vector expressing system LV-luciferase (provided by Dr. Lung-Ji Chang, Department of Molecular Genetics and Microbiology, University of Florida) and selected for stabilized expressing clones by series dilution selection. The stabilized expressing luciferase cell line was renamed SUP-LUC2.

Hu Z, & Slayton W.B. (2014). Integrin VLA-5 and FAK are Good Targets to Improve Treatment Response in the Philadelphia Chromosome Positive Acute Lymphoblastic Leukemia. Frontiers in Oncology, 4, 112.

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