Conventional formulation of metribuzin (Sencor® 480), technical metribuzin (95% purity), and 14C-metribuzin (98% purity and 2.3 Bq mg−1 of specific activity, American Radiolabeled Chemicals, Inc., St. Louis, MO, USA) and Scintillation solution (Ultima Gold, PerkinElmer, Waltham, MA, USA) were used in the studies. In nanoparticle preparation, caprylic/capric triglyceride (Myritol 318) was purchased from Basf (Basf Co. Ltd., São Paulo, SP, Brazil), poly-ε-caprolactone (PCL) (Mn∼80,000 Da), polysorbate 80 (Mn∼1310 Da), and sorbitane monostearate (Mw = 430.63 g mol−1) were purchased from Sigma Aldrich (Sigma-Aldrich, Chem. Co., St. Louis, MO, USA). Soil samples were collected in Paraná and São Paulo State and organic residues were collected in agricultural areas.
14c metribuzin
Manufactured by American Radiolabeled Chemicals
Sourced in United States, Sao Tome and Principe
14C-metribuzin is a radiolabeled chemical compound used for research and analytical purposes. It contains the radioactive carbon-14 isotope, which can be used to track and quantify the compound in various experimental settings. The core function of 14C-metribuzin is to serve as a research tool for studying the behavior, fate, and metabolism of metribuzin, a herbicide, in biological systems.
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2 protocols using 14c metribuzin
1
Radioactive Metribuzin Nanoparticle Formulation
2
Metribuzin Formulation and Enzyme Assays
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Polycaprolactone (Mn ∼
80,000 Da), sorbitane monostearate (Mw: 430.63 g mol–1), and polysorbate 80 (Mn ∼ 1310 Da) were purchased from Sigma-Aldrich
(Sigma-Aldrich, Chem. Co.) and caprylic/capric triglyceride (Myritol
318) from Basf Co. Ltd, Brazil. Technical-grade metribuzin (95%) and
the conventional formulation (Sencor 500 CG), 14C-metribuzin
(95%) with a specific activity of 2.3 Bq mg–1 (American
Radiolabeled Chemicals, Inc., St. Louis, MO), and scintillation solution
(Ultima Gold, PerkinElmer, Waltham, MA) were also used. Acetone ACS
(>99.5%) and high-performance liquid chromatography (HPLC)-grade
methanol
were purchased from Dinâmica Co. Ltd, Brazil.
The substrates
(4-methylumbelliferone-phosphate (Mub-P), 4-methylumbelliferone-glucopyranoside
(Mub-C), and 4-methylumbelliferone-sulfate (Mub-S)) and fluorophore
(4-methylumbelliferone, Mub) for the enzyme assays were purchased
from Sigma-Aldrich. Other reagents (analytical grade or better) were
purchased from local suppliers.
80,000 Da), sorbitane monostearate (Mw: 430.63 g mol–1), and polysorbate 80 (Mn ∼ 1310 Da) were purchased from Sigma-Aldrich
(Sigma-Aldrich, Chem. Co.) and caprylic/capric triglyceride (Myritol
318) from Basf Co. Ltd, Brazil. Technical-grade metribuzin (95%) and
the conventional formulation (Sencor 500 CG), 14C-metribuzin
(95%) with a specific activity of 2.3 Bq mg–1 (American
Radiolabeled Chemicals, Inc., St. Louis, MO), and scintillation solution
(Ultima Gold, PerkinElmer, Waltham, MA) were also used. Acetone ACS
(>99.5%) and high-performance liquid chromatography (HPLC)-grade
methanol
were purchased from Dinâmica Co. Ltd, Brazil.
The substrates
(4-methylumbelliferone-phosphate (Mub-P), 4-methylumbelliferone-glucopyranoside
(Mub-C), and 4-methylumbelliferone-sulfate (Mub-S)) and fluorophore
(4-methylumbelliferone, Mub) for the enzyme assays were purchased
from Sigma-Aldrich. Other reagents (analytical grade or better) were
purchased from local suppliers.
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