Antioxidant activity was determined by DPPH radical-scavenging capacity, ferric reducing antioxidant power (FRAP) and ABTS (2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)) assay as described by Belščak et al. (25 (link)) with some modifications. For the DPPH assay, 0.02 mL of the extract was added to 0.95 mL or 0.06 mM DPPH (2,2-diphenyl-picrylhidrazyl; Sigma Aldrich, Merck) in methanol (HPLC ultra gradient, J.T.Baker). The free radical-scavenging capacity was evaluated by measuring the absorbance at 517 nm (Specord 50 Plus; Analytik Jena) after a 30-minute reaction in the dark. For FRAP assay, 0.01 mL of methanol extract was mixed with 1 mL of FRAP reagent. Absorbance was measured at 593 nm after incubation for 4 min. For the ABTS assay, 0.02 mL of extract was mixed with 2 mL of ABTS reagent (Sigma-Aldrich, Merck), mixed, and the absorbance measured at 734 nm after 6 min of incubation in the dark. Measurements were done in triplicate and the results were expressed in µmol Trolox equivalent (TE) per gram of sample, using a calibration curve constructed from the standard solution of Trolox (>97%; Sigma-Aldrich, Merck).
Abts reagent
Manufactured by Merck Group
Sourced in United States, Germany
The ABTS reagent is a colorimetric detection reagent used in enzymatic assays. It is a substrate for various enzymes, including peroxidases and oxidases, which catalyze its oxidation to a colored product. The ABTS reagent is commonly used in immunoassays, enzyme-linked immunosorbent assays (ELISA), and other analytical applications that require colorimetric detection.
Automatically generated - may contain errors
Lab products found in correlation
Trolox, by Merck Group (3 mentions)
Dpph reagent, by Merck Group (3 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
Specord 50 plus, by Analytik Jena (1 mentions)
Folin ciocalteu reagent, by Solarbio (1 mentions)
Mili q, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Sodium dihydrogen phosphate dihydrate, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Potato dextrose broth (pdb), by Merck Group (1 mentions)
Potassium persulphate, by Thermo Fisher Scientific (1 mentions)
Mueller hinton broth (mhb), by Merck Group (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions)
Mueller hinton agar (mha), by Merck Group (1 mentions)
2 4 6 tris 2 pyridyl 1 3 5 triazine tptz, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Thermo Fisher Scientific (1 mentions)
9 protocols using abts reagent
1
Antioxidant Activity Evaluation Protocols
2
Quantifying Antibody Responses to Viral and Helminth Antigens
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibody levels in sera and BAL were detected by ELISA. Briefly, plates were coated with antigen or anti-mouse pan-Ig antibody and then serial dilutions of serum or BAL fluid added to the plates. The bound antibody was then detected using a SBA Clonotyping System-B6/C57J-HRP kit (Southern Biotech) and ABTS reagent (Sigma-Aldrich). We then calculated the dilution of serum or BAL which gave 50% of the maximal responses using GraphPad.
Flu-specific antibodies were coating a plate with 500 HAU ml−1 heat-killed Influenza A PR8; NP-specific responses were detected using NP-BSA (Biosearch) coated plates. HES-specific responses were detected by coating a plate with 1 µgml−1 HES (a kind gift of Rick Maizels, Edinburgh, UK).
Flu-specific antibodies were coating a plate with 500 HAU ml−1 heat-killed Influenza A PR8; NP-specific responses were detected using NP-BSA (Biosearch) coated plates. HES-specific responses were detected by coating a plate with 1 µgml−1 HES (a kind gift of Rick Maizels, Edinburgh, UK).
3
Xanthoceras sorbifolia Husk Antioxidant Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SPI was bought from Shansong Biological Products Co., Ltd. (Linyi, China). Xanthoceras sorbifolia husks were collected in August 2021 in Xinzhou city, Shanxi Province. Sodium hydroxide (NaOH) and potassium carbonate (K2CO3) were provided by Guanghua Sci-Tech Co., Ltd. (Guangzhou, China). Ethanol and glycerol were ordered from Kelong Chemical Co., Ltd. (Chengdu, China). The ABTS reagent (2,2′-azino-bis [3-ethylbenzothiazoline-6-sulfonic acid] diammonium salt) was provided by Sigma‒Aldrich Inc. (Saint Louis, MO, USA). The DPPH reagent (2,2-diphenyl-1-picrylhydrazyl) was purchased from Alfa Aesar (Ward Hill, MA, USA). The Folin–Ciocalteu reagent was provided by Solarbio Inc. (Beijing, China).
4
Antioxidant Activity Determination by ABTS Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antioxidative activity was determined against the ABTS reagent, according to the method described by Re et al. 1999 [29 (link)]. In briefl 3 mL of ABTS reagent (Sigma-Aldrich, Munich, Germany) and 30 µL of sample were measured into 10 mL test tubes. The tubes were tightly closed, mixed thoroughly, and set aside in the dark for 6 min at 21 °C. After the incubation, spectrophotometric analysis (Halo SB-10 Dynamica Biogenet, Cambridge, UK) was performed at 735 nm. Results of the assay were expressed as the capability of antioxidants to scavenge ABTS radicals, relative to that of Trolox (Sigma-Aldrich, Munich, Germany), and were presented as mM of Trolox equivalent per liter of wine (mM TE/L).
5
Comparative Analysis of Wheat and Spelt
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Wheat (Triticum aestivum cv. Chimaboo) and spelt (Triticum spelta cv. Ostro) seeds were provided by the local milling industry. Seeds were harvested in Slovenia and stored at 1 °C under darkness until used for analyses. Refined wheat flour (type 500) was obtained from a local store. Folin–Ciocalteu reagent, methanol (99.9%), and sodium dihydrogen phosphate dihydrate were obtained from Merck (Darmstadt, Germany). ABTS reagent, DPPH reagent, hydrochloric acid, manganese (VI) oxide, sodium carbonate, and Trolox were purchased from Sigma-Aldrich (Steinheim, Germany). Sodium hydroxide was purchased from Honeywell Fluka (Seelze, Germany). All of the reagents were of analytical quality. Ultrapure water (MiliQ, Millipore, Burlington, MA, USA) was used for the preparation of solutions.
6
Assessing Antioxidant Activity of Chitosan/PLA Films
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antioxidant activity of the chitosan/PLA bilayer films was tested using 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) reagent. The assay is based on the spectrophotometric determination (UV-VIS) of the decolorization of the reagent in the presence of an antioxidant. The ABTS reagent (7 mM; Sigma-Aldrich, St. Louis, MO, USA) was prepared in 2.45 mM potassium persulfate (Sigma-Aldrich, St. Louis, MO, USA) and diluted with phosphate-buffered saline (PBS; Gibco, Life Technologies, Grand Island, NY, USA). Absorbance was measured at 734 nm and 25 °C at time points of 0 min, 15 min, and 60 min after addition of 0.1 g of film sample to 3.9 mL of ABTS solution. The solution was shaken during the extraction. The results are given as percentage of inhibition [18 (link)].
7
Antioxidant, Cytotoxicity, and Antimicrobial Activities
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Glacial acetic acid, methanol, and sodium acetate were purchased from QReC, Selangor, Malaysia. DPPH, 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), ABTS reagent, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent, iron(II) sulphate (FeSO4.7H2O), and BHA were obtained from Sigma-Aldrich, Missouri, USA. Mueller Hinton Agar (MHA), Mueller Hinton Broth (MHB), Potato Dextrose Broth (PDB), Sabouraud Dextrose Agar (SDA), and commercial silver nanoparticles (C-AgNP) were acquired from Merck, Darmstadt, Germany. Gentamycin sulphate, nystatin, and hydrochloric acid (HCl) were provided by Biobasic, Ontario, Canada. Glycerol was obtained from System®, Selangor, Malaysia. Potassium persulphate was purchased from Acros Organics, New Jersey, USA. 0.25% Trypsin-EDTA, RPMI 1640, penicillin-streptomycin, foetal bovine serum (FBS), L-glutamine, and Dulbecco's Modified Eagle Medium (DMEM) were acquired from Gibco®, New York, USA. 5-fluorouracil (5-FU) was purchased from Tokyo Chemical Industry, Tokyo, Japan. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) kit was provided by Promega, WI, USA. Phosphate buffer saline (PBS) was obtained from Invitrogen, New York, USA.
8
Brewing Coffee Roast Diversity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BSGs with different roasting levels (Pilsen, Caramel 60, Caramel 120 and Chocolate) were obtained in the laboratory under equivalent industrial conditions (Palmer, 2006) . Sodium carbonate, sodium acetate and acetic acid were supplied by Biopack. DPPH reagent, gallic acid, Folin-Ciocalteu reagent, 6-Hydroxy-2,5,7,8-tetramethylchromane-2carboxylic acid (Trolox standard), ABTS reagent and potassium persulphate were purchased from Sigma-Aldrich, while TPTZ reagent was supplied by Fluka Chemicals. All these chemicals were of analytical grade.
9
Cyanidin Antioxidant Activity Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antioxidant activity of the cyanidin was measured, using a modified ABTS radical-scavenging activity assay, as reported by Re et al. 27 The ABTS reagent (Sigma, St. Louis, MO) was dissolved in distilled water until the absorbance reached 1.7. A total of 90 ml of the ABTS stock solution was added to each 10 ml concentration of cyanidin (Enzo Life Science, Farmingdale, NY) in a 96-well plate. The plate was shaken to ensure thorough mixing before being wrapped in foil and placed in the dark for 10 min. Then, the absorbance of the mixture was measured, using an ELISA reader at a wavelength of 415 nm.
The antioxidant activity of the cyanidin was also measured, using a modified DPPH radical-scavenging assay. 28 The DPPH reagent (Sigma, St. Louis, MO) was dissolved in ethanol to prepare the concentration of 5 mg/ml solution. 90 ml of the DPPH stock solution was added to each 10 ml concentration of cyanidin in a 96-well plate. The plate was shaken to ensure thorough mixing before being wrapped in foil and placed in the dark for 30 min. After that, the absorbance of the mixture was measured, using an ELISA reader at a wavelength of 517 nm. A well-known ascorbic acid was used as a positive control in both antioxidant activity assays.
The antioxidant activity of the cyanidin was also measured, using a modified DPPH radical-scavenging assay. 28 The DPPH reagent (Sigma, St. Louis, MO) was dissolved in ethanol to prepare the concentration of 5 mg/ml solution. 90 ml of the DPPH stock solution was added to each 10 ml concentration of cyanidin in a 96-well plate. The plate was shaken to ensure thorough mixing before being wrapped in foil and placed in the dark for 30 min. After that, the absorbance of the mixture was measured, using an ELISA reader at a wavelength of 517 nm. A well-known ascorbic acid was used as a positive control in both antioxidant activity assays.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!