After being washed with fresh Ringer's solution, the coverslips were mounted on a recording chamber. Imaging was carried out at room temperature on an inverted fluorescence microscope (IMT-Olympus, Tokyo, Japan) equipped with a SIT camera (C10600, Hamamatsu Photonics, Hamamatsu, Japan), a Lambda XL light source (Sutter Instrument, Novato, CA, USA), and Lamba-10B optical filter changer (Sutter Instrument). Using a 1260 Infinity HPLC system (Agilent Technologies, Santa Clara, CA, USA) the dissociated OSNs were stimulated with the odorants in random order between two flanking stimulations with the lead odorant, [1]. A final stimulation with a 10 μM Forskolin (Sigma-Aldrich) solution was made to assess the viability of the OSNs. Recordings were made at 490 nm excitation and 520 nm emission. Images were taken every 4 s and there was a 4 min delay between stimulations. The images were then computed using Metamorph Premier software (Molecular Device LLC, Downingtown, PA, USA) and the cells were manually counted.
Sit camera c10600
Manufactured by Hamamatsu Photonics
Sourced in Japan
The SIT camera (C10600) is a scientific imaging camera developed by Hamamatsu Photonics. It utilizes a Silicon Intensified Target (SIT) sensor to capture images. The core function of the SIT camera is to provide high-sensitivity image detection for applications that require low-light imaging.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sit camera c10600
1
Odorant-Induced Calcium Imaging of Olfactory Sensory Neurons
2
Calcium Imaging of Odorant Responses in Dissociated Olfactory Sensory Neurons
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After being washed with fresh Ringer’s solution, the coverslips were mounted on a recording chamber. Imaging was carried out at room temperature on an inverted fluorescence microscope (IMT-Olympus) equipped with an SIT camera (C10600, Hamamatsu Photonics), a Lambda XL light source (Sutter Instrument), and Lamba-10B optical filter changer (Sutter Instrument). Using a 1260 Infinity HPLC system (Agilent Technologies), the dissociated OSNs were stimulated with the odorants in random order. A final stimulation with a 10 μM forskolin (Sigma-Aldrich) solution was made to assess the viability of the OSNs. Recordings were made at 490-nm excitation and 520-nm emission. Images were taken every 4 s, and there was a 4-min delay between stimulations. The images were then computed using MetaMorph Premier software (Molecular Devices LLC), and the cells were manually counted. Cells exhibiting an intensity increase of at least 10% ΔF/F0 amplitude between 8 and 12 frames after the odorant injection were considered responsive cells.
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