Centricon plus 70 100 kda centrifugal filter devices

Manufactured by Merck Group

The Centricon Plus-70 100-kDa Centrifugal Filter Devices are laboratory equipment used for sample concentration and buffer exchange. They utilize centrifugal force to filter and concentrate macromolecules such as proteins, peptides, and nucleic acids with a molecular weight cutoff of 100 kDa.

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Lab products found in correlation

Amicon ultra 15 100 kda centrifugal filter devices, by Merck Group (2 mentions) Boric acid, by Thermo Fisher Scientific (1 mentions) Trizma ph 9, by Merck Group (1 mentions)

Close spelling variants of this product

Centricon Plus-70 filter device 100 kDa centrifugal filter device Centricon Plus‐70 Centrifugal Filter Centricon® centrifugal filter devices Centricon Plus-70 filter Centricon Centrifugal filter Centrifugal filter devices Centricon Plus-70 Centricon device 100 KDa filter

2 protocols using centricon plus 70 100 kda centrifugal filter devices

Concentrating Cell Culture Supernatants

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Inactivated cell culture supernatants were concentrated in Amicon Ultra-15 100-kDa Centrifugal Filter Devices (Millipore) or Centricon Plus-70 100-kDa Centrifugal Filter Devices at 3500 x g for 10–45 min at 4°C. Any material that was inadvertently concentrated beyond the desired concentration factor was diluted to the correct volume with 0.1M trizma/BS buffer or 1X PBS.

Goodman C.H., Russell B.J., Velez J.O., Laven J.J., Nicholson W.L., Bagarozzi DA J.r., Moon J.L., Bedi K, & Johnson B.W. (2014). Development of an algorithm for production of inactivated arbovirus antigens in cell culture. Journal of virological methods, 208, 66-78.

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Antigen Concentration and Inactivation

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Antigen was concentrated after inactivation, as it had been determined empirically that antigen activity was lost if it was concentrated before inactivation. Inactivated cell culture supernatants were concentrated in Amicon Ultra-15 100kDa Centrifugal Filter Devices (Millipore, Billerica, MA) or Centricon Plus-70 100-kDa Centrifugal Filter Devices (Millipore) at 3500 x g for 10-45 min at 4°C. The final volume was adjusted with 0.1M trizma/BS buffer: 1.0M trizma pH 9.0 (Sigma-Aldrich) + borate saline solution pH 9.0 [1.5M sodium chloride (Daigger, Vernon Hills, IL), 0.5M boric acid (Fisher Scientific), 1.0N sodium hydroxide (Daigger)] to the desired concentration factor.

Goodman C.H., Russell B.J., Velez J.O., Laven J.J., Bagarozzi DA J.r., Moon J.L., Bedi K, & Johnson B.W. (2015). Production of a Sindbis/Eastern Equine Encephalitis Chimeric Virus Inactivated Cell Culture Antigen. Journal of virological methods, 223, 19-24.

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