The levels of TNF-α and IL-6 were measured using a rat TNF-α ELISA kit (catalog No: K1052-100; Biovision, San Francisco, CA, USA) and a rat IL-6 ELISA kit (catalog No: K4145-100; Biovision), respectively. The contents of creatine kinase (CK)-MB and cardiac troponin I (cTnI) in the serum of rats were measured using a CK-MB isoenzyme Assay Kit (catalog No: H197-1-1; Nanjing Jiancheng Bioengineering, Inc., Nanjing, China) and a rat Cardiac Troponin I ELISA ELISA kit (catalog No: ab246529; Abcam, Cambridge, MA, USA), respectively.
Ck mb isoenzyme assay kit
Manufactured by Nanjing Jiancheng
Sourced in China
The CK-MB isoenzyme Assay Kit is a laboratory diagnostic tool used to measure the levels of the CK-MB isoenzyme, which is a specific type of creatine kinase found in the heart muscle. This assay provides a quantitative determination of CK-MB concentration in a sample, which can be useful in the diagnosis and monitoring of certain heart-related conditions.
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3 protocols using ck mb isoenzyme assay kit
1
Evaluating Cardiac and Inflammatory Markers
2
Measuring LDH and CK-MB Biomarkers
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LDH activity in the NRCM and H9C2 cell culture medium, and the activities of LDH and CK-MB in the mouse serum were measured using an LDH assay kit (Jiancheng, A020) and CK-MB isoenzyme Assay Kit (Jiancheng, H197) according to the manufacturer's instructions, respectively [15 (link)].
3
Serum Biomarkers After IR Surgery
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One day after the IR surgery, the mice were anesthetized, and blood samples were collected from the orbital venous plexus. Serum levels of CK, CKMB, and LDH were determined with a mouse CK assay kit, a CKMB isoenzyme assay kit, and an LDH Cytotoxicity Assay Kit (Nanjing Jiancheng Bioengineering Institute. Nanjing, China), respectively. The optical density(OD) of each sample was measured with a microplate reader (Agilent Technologies, California, USA). The CK assay was read at a wavelength of 660 nm, the CKMB assay at 340 nm, and the LDH assay at 490 nm.
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