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2 protocols using ceritinib

1

Cell Culture Maintenance and Compound Screening

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All cell lines were maintained in a humidified incubator at 37 °C with 5% CO2. PC9, HCC827, H3122, A549, A375, GR4, WZR12, PC9R, MGH134, MGH006, MGH1109, and MOLM13 were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MIA PaCa-2 and SW1573 were cultured in DMEM/F-12 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MGH119 were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. 293FT cells were cultured in DMEM high glucose medium with 10% FBS, 1% penicillin–streptomycin, 1% sodium pyruvate, 1% nonessential amino acids and 1% GlutaMax. All cell lines were purchased from American Type Culture Collection or Duke University Cell Culture Facility except for MGH lines, which were obtained from Dr. Aaron Hata. Cell lines were authenticated using the Promega PowerPlex 18D kit. Drugs were purchased from ApexBio (Osimertinib, Gefitinib, SCH772984, Ceritinib, Quizartinib, PLX4720, AMG510, Lorlatinib), Cayman Chemical (Olaparib), and SelleckChem (AZD0156, AZD1390, Q-VD-Oph, Cycloheximide).
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2

Cell Culture Maintenance and Compound Screening

Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell lines were maintained in a humidified incubator at 37 °C with 5% CO2. PC9, HCC827, H3122, A549, A375, GR4, WZR12, PC9R, MGH134, MGH006, MGH1109, and MOLM13 were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MIA PaCa-2 and SW1573 were cultured in DMEM/F-12 medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. MGH119 were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin. 293FT cells were cultured in DMEM high glucose medium with 10% FBS, 1% penicillin–streptomycin, 1% sodium pyruvate, 1% nonessential amino acids and 1% GlutaMax. All cell lines were purchased from American Type Culture Collection or Duke University Cell Culture Facility except for MGH lines, which were obtained from Dr. Aaron Hata. Cell lines were authenticated using the Promega PowerPlex 18D kit. Drugs were purchased from ApexBio (Osimertinib, Gefitinib, SCH772984, Ceritinib, Quizartinib, PLX4720, AMG510, Lorlatinib), Cayman Chemical (Olaparib), and SelleckChem (AZD0156, AZD1390, Q-VD-Oph, Cycloheximide).
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